10.1371/journal.pgen.1005207.g005
Eduardo Flores-Sandoval
Eduardo
Flores-Sandoval
D. Magnus Eklund
D.
Magnus Eklund
John L. Bowman
John
L. Bowman
Developmental effects of auxin insensitivity.
Public Library of Science
2015
tpl
polymorpha life cycle
acid
basal lineages share
AUXIN RESPONSE FACTOR
flowering plant systems
transcription factors
liverwort Marchantia polymorpha
transcriptional regulation acts
MpTRANSPORT INHIBITOR RESPONSE 1 auxin receptor
plant hormone auxin
Simple Auxin Transcriptional Response System Regulates Multiple Morphogenetic Processes
auxin transcriptional response machinery
land plants
totipotent cell states
MpARF
arf
2015-05-28 03:33:31
Figure
https://plos.figshare.com/articles/figure/_Developmental_effects_of_auxin_insensitivity_/1429263
<p><b>(A)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>IAA</i></sup> lines suffer from severe dwarfism compared to <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL</i> plants. <b>(B)</b> Strong <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>IAA</i></sup> line showing a lack of organized patterning, dorsiventral polarity and a limited degree of differentiation. Scales (arrowhead), air pores (asterisk) and rhizoids (r) are visible in random places and growth is severely affected. <b>(C)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF1</i></sup> representative line showing a degree of differentiation similar to the prothallus stage of wild-type development. <b>(D)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF1</i></sup> showing single cell layer prothalli-like tisssue. <b>(E)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF2</i></sup> representative line. <b>(F)</b> Wild-type sporeling (10 days) transitioning into a single cell layered prothallus (14 days) and fully established multiple prothalli 18 days after plating. <b>(G)</b> Strong <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>BDL</i></sup> line lacking growth, differentiation and dorsiventrality, but still capable of producing rhizoids. <b>(H)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF3</i></sup> lines differentiate gemmae cups and a dorsiventral polarity with dorsal air chambers and ventral rhizoids. The thallus has an apparent hyponastic curvature. <b>(I, J)</b> Multiple independent Hyg<sup>Res</sup> sporelings (transformed with an empty binary vector) grown on media containing 10μg/ml Hyg and mock (<b>I</b>) or 10μM 2,4-D (<b>J</b>) for 14 days. <b>(K, L)</b> Multiple independent <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpIAA</i></sup> (<b>K</b>) or <sub><i>pro</i></sub><i>MpTPL</i>:<i>MpTPL-D34</i><sup><i>MpIAA</i></sup> (<b>L</b>) lines grown on 10μM 2,4-D for 14 days. Scale bars in C, E, I, J, K and L = 1mm; A = 1cm; B = 430μm; D = 130μm; F = 30μm; G = 600μm; H = 860μm.</p>