10.1371/journal.pgen.1005207.g005 Eduardo Flores-Sandoval Eduardo Flores-Sandoval D. Magnus Eklund D. Magnus Eklund John L. Bowman John L. Bowman Developmental effects of auxin insensitivity. Public Library of Science 2015 tpl polymorpha life cycle acid basal lineages share AUXIN RESPONSE FACTOR flowering plant systems transcription factors liverwort Marchantia polymorpha transcriptional regulation acts MpTRANSPORT INHIBITOR RESPONSE 1 auxin receptor plant hormone auxin Simple Auxin Transcriptional Response System Regulates Multiple Morphogenetic Processes auxin transcriptional response machinery land plants totipotent cell states MpARF arf 2015-05-28 03:33:31 Figure https://plos.figshare.com/articles/figure/_Developmental_effects_of_auxin_insensitivity_/1429263 <p><b>(A)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>IAA</i></sup> lines suffer from severe dwarfism compared to <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL</i> plants. <b>(B)</b> Strong <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>IAA</i></sup> line showing a lack of organized patterning, dorsiventral polarity and a limited degree of differentiation. Scales (arrowhead), air pores (asterisk) and rhizoids (r) are visible in random places and growth is severely affected. <b>(C)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF1</i></sup> representative line showing a degree of differentiation similar to the prothallus stage of wild-type development. <b>(D)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF1</i></sup> showing single cell layer prothalli-like tisssue. <b>(E)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF2</i></sup> representative line. <b>(F)</b> Wild-type sporeling (10 days) transitioning into a single cell layered prothallus (14 days) and fully established multiple prothalli 18 days after plating. <b>(G)</b> Strong <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>BDL</i></sup> line lacking growth, differentiation and dorsiventrality, but still capable of producing rhizoids. <b>(H)</b><sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpARF3</i></sup> lines differentiate gemmae cups and a dorsiventral polarity with dorsal air chambers and ventral rhizoids. The thallus has an apparent hyponastic curvature. <b>(I, J)</b> Multiple independent Hyg<sup>Res</sup> sporelings (transformed with an empty binary vector) grown on media containing 10μg/ml Hyg and mock (<b>I</b>) or 10μM 2,4-D (<b>J</b>) for 14 days. <b>(K, L)</b> Multiple independent <sub><i>pro</i></sub><i>EF1</i>:<i>MpTPL-D34</i><sup><i>MpIAA</i></sup> (<b>K</b>) or <sub><i>pro</i></sub><i>MpTPL</i>:<i>MpTPL-D34</i><sup><i>MpIAA</i></sup> (<b>L</b>) lines grown on 10μM 2,4-D for 14 days. Scale bars in C, E, I, J, K and L = 1mm; A = 1cm; B = 430μm; D = 130μm; F = 30μm; G = 600μm; H = 860μm.</p>