Identification of critical residues in the Mib1-Interacting Domain (MID). Gregory Palardy Ajay B. Chitnis 10.1371/journal.pone.0127864.g002 https://plos.figshare.com/articles/figure/_Identification_of_critical_residues_in_the_Mib1_Interacting_Domain_MID_/1429262 <p>(A) Conserved amino acids (pink shading) in the putative <i>Mib1</i>-Interacting Domain in Delta ligands. (B) KK and NN/KK mutants do not significantly interact with mind bomb. DeltaD-HA, ∆B and point mutation (NN, KK and NN/KK) constructs are co-immunoprecipitated with Myc-Mib1 using anti-Myc Ab and detected with anti-HA Ab. (C) KK and NN/KK mutants are not significantly ubiquitylated by Mib1. DeltaD-HA, ∆B and point mutation constructs co-transfected with Flag-Ubiquitin (Flag-Ub) with and without Myc-Mib1 are immunoprecipitated with anti-HA Ab and detected with Anti-Flag Ab to assay ubiquitylation of full length and mutant forms of DeltaD.</p> 2015-05-28 03:35:38 mid bomb 1 Interaction Domain mib 1 Interaction Domain RING E 3 ligase zebrafish Notch ligand DeltaD neurogenic phenotype Zebrafish DeltaD Ubiquitylation