10.1371/journal.pgen.1005265.g003
Dena H. S. Block
Dena
H. S. Block
Kwame Twumasi-Boateng
Kwame
Twumasi-Boateng
Hae Sung Kang
Hae
Sung Kang
Jolie A. Carlisle
Jolie
A. Carlisle
Alexandru Hanganu
Alexandru
Hanganu
Ty Yu-Jen Lai
Ty
Yu-Jen Lai
Michael Shapira
Michael
Shapira
<i>elt-2</i> is essential for <i>atf-7</i>-dependent immune gene regulation.
Public Library of Science
2015
function
elegans larvae
elegans GATA transcription factors
SKN
role
atf
infection resistance
elt
elegans immunity
survival assays
hydrolytic enzymes
adult intestine
Pseudomonas aeruginosa Infection
Adult C
terminal differentiation
microarray analysis
gene
2015-05-27 03:44:27
Figure
https://plos.figshare.com/articles/figure/_elt_2_is_essential_for_atf_7_dependent_immune_gene_regulation_/1428052
<p><i>A</i>. Survival curves for wildtype and <i>atf-7(qd22qd130)</i> animals fed with designated RNAi’s during development followed by infection. Averages ± SDs for three plates (N = 129–140 per group) in a representative experiment of several others with similar results. <i>B-E</i>, Gene expression (log scale) in wildtype, <i>pmk-1(km25)</i>, and <i>atf-7(qd22qd130)</i> animals, fed with designated RNAi’s during development. Models depict for each panel the mode of disruption, or status, of examined factors (solid-line crosses, loss-of-function mutants; dashed-line crosses, knock-down), placing ELT-2 tentatively at the proximal promoter of immune genes (Gene X) putatively regulated by PMK-1 and ATF-7; <i>atf-7</i> is depicted as an activator (arrow) or repressor (blunt-ended arrow), based on disruption effects on gene expression. RNA levels were measured in L4/YA worms. Each panel presents averages and SDs for two independent experiments. Asterisks mark inter-group significance with *p<0.05, **p<0.01, and NS, non-significant (paired t-test) for all genes in the group or in underlined subset.</p>