%0 Figure %A Khan, Mahmood %A Xu, Yanyi %A Hua, Serena %A Johnson, Jed %A Belevych, Andriy %A M. L. Janssen, Paul %A Gyorke, Sandor %A Guan, Jianjun %A G. Angelos, Mark %D 2015 %T Electrical properties of hiPSC-CMs. %U https://plos.figshare.com/articles/figure/_Electrical_properties_of_hiPSC_CMs_/1420073 %R 10.1371/journal.pone.0126338.g007 %2 https://plos.figshare.com/ndownloader/files/2074697 %K alignment %K cardiomyocyte %K scanning electron microscopy %K decellularized rat myocardium %K Human Induced Pluripotent Stem Cell Derived Cardiomyocytes %K measure calcium transients %K cell therapy %K tissue culture plate %K sem %K plga %K surface culture plates %K nanofiber patch %X

A) Representative action potential (AP) traces recorded during 1 Hz stimulation in patch-clamped single human iPS-CM seeded on flat bottom or on aligned nanofiber-coated coverslips. Dotted lines indicate 0 mV level. B) Summary graphs showing average data for AP amplitude and AP duration at 50 (APD50) and 90 (APD90) % repolarization levels, respectively. Data was recorded in cells from flat plate (Flat, n = 5) and aligned nanofiber (Nano, n = 4) groups, respectively. Resting potential was -74± 2 mV in flat bottom, and -74± 1 mV in aligned nanofiber groups. C) Representative action potential (AP) traces recorded in syncytium of human iPS-CM seeded on flat bottom or on aligned nanofiber-coated coverslips. APs were evoked by electrical field stimulation at 1 Hz and were recorded using voltage-sensitive dye di-4-AN(F)EPPTEA. D) Summary graphs showing average data for AP amplitude and AP duration. Data was obtained both flat plate (Flat, n = 6) and aligned nanofiber (Nano, n = 6) preparations, respectively.

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