Electrical properties of hiPSC-CMs.
Mahmood Khan
Yanyi Xu
Serena Hua
Jed Johnson
Andriy Belevych
Paul M. L. Janssen
Sandor Gyorke
Jianjun Guan
Mark G. Angelos
10.1371/journal.pone.0126338.g007
https://plos.figshare.com/articles/figure/_Electrical_properties_of_hiPSC_CMs_/1420073
<p><b>A)</b> Representative action potential (AP) traces recorded during 1 Hz stimulation in patch-clamped single human iPS-CM seeded on flat bottom or on aligned nanofiber-coated coverslips. Dotted lines indicate 0 mV level. <b>B)</b> Summary graphs showing average data for AP amplitude and AP duration at 50 (APD50) and 90 (APD90) % repolarization levels, respectively. Data was recorded in cells from flat plate (Flat, n = 5) and aligned nanofiber (Nano, n = 4) groups, respectively. Resting potential was -74± 2 mV in flat bottom, and -74± 1 mV in aligned nanofiber groups. <b>C)</b> Representative action potential (AP) traces recorded in syncytium of human iPS-CM seeded on flat bottom or on aligned nanofiber-coated coverslips. APs were evoked by electrical field stimulation at 1 Hz and were recorded using voltage-sensitive dye di-4-AN(F)EPPTEA. <b>D)</b> Summary graphs showing average data for AP amplitude and AP duration. Data was obtained both flat plate (Flat, n = 6) and aligned nanofiber (Nano, n = 6) preparations, respectively.</p>
2015-05-19 03:12:13
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