Electrical properties of hiPSC-CMs. Mahmood Khan Yanyi Xu Serena Hua Jed Johnson Andriy Belevych Paul M. L. Janssen Sandor Gyorke Jianjun Guan Mark G. Angelos 10.1371/journal.pone.0126338.g007 https://plos.figshare.com/articles/figure/_Electrical_properties_of_hiPSC_CMs_/1420073 <p><b>A)</b> Representative action potential (AP) traces recorded during 1 Hz stimulation in patch-clamped single human iPS-CM seeded on flat bottom or on aligned nanofiber-coated coverslips. Dotted lines indicate 0 mV level. <b>B)</b> Summary graphs showing average data for AP amplitude and AP duration at 50 (APD50) and 90 (APD90) % repolarization levels, respectively. Data was recorded in cells from flat plate (Flat, n = 5) and aligned nanofiber (Nano, n = 4) groups, respectively. Resting potential was -74± 2 mV in flat bottom, and -74± 1 mV in aligned nanofiber groups. <b>C)</b> Representative action potential (AP) traces recorded in syncytium of human iPS-CM seeded on flat bottom or on aligned nanofiber-coated coverslips. APs were evoked by electrical field stimulation at 1 Hz and were recorded using voltage-sensitive dye di-4-AN(F)EPPTEA. <b>D)</b> Summary graphs showing average data for AP amplitude and AP duration. Data was obtained both flat plate (Flat, n = 6) and aligned nanofiber (Nano, n = 6) preparations, respectively.</p> 2015-05-19 03:12:13 alignment cardiomyocyte scanning electron microscopy decellularized rat myocardium Human Induced Pluripotent Stem Cell Derived Cardiomyocytes measure calcium transients cell therapy tissue culture plate sem plga surface culture plates nanofiber patch