%0 Figure %A K. Sack, Brandon %A J. Keitany, Gladys %A M. Vaughan, Ashley %A L. Miller, Jessica %A Wang, Ruobing %A H. I. Kappe, Stefan %D 2015 %T Antibodies elicited by LAGAP in C57BL/6 but not BALB/cJ mice are of broad isotypes and recognize the merozoite surface. %U https://plos.figshare.com/articles/figure/_Antibodies_elicited_by_LAGAP_in_C57BL_6_but_not_BALB_cJ_mice_are_of_broad_isotypes_and_recognize_the_merozoite_surface_/1414854 %R 10.1371/journal.ppat.1004855.g005 %2 https://plos.figshare.com/ndownloader/files/2067819 %K erythrocytic stages %K erythrocytic parasite life cycle stages %K liver stage development %K sporozoite challenge %K merozoite surface %K B cell targets %K complement fixation %K immunization strategy %K attenuated parasites %K blood stage infection %K malaria vaccine development efforts %K life cycle stages %K liver stages %K GAP immunization %K Plasmodium parasite infection %K Plasmodium yoelii %K blood stage challenge %K life cycle %K FC receptor binding %K parasite transmission %K blood stage parasites %X

Serum was collected from LAGAP immunized C57BL/6 (top 3 rows) and BALB/cJ (bottom 3 rows) mice as in Fig 4 and used in a blood stage IFA. Secondary antibodies against total IgG, IgG1 and IgG2b were used to visualize isotype-specific anti-blood stage antibodies. Parasites were also visualized with antibodies recognizing MSP1 and DNA visualized with 4',6-diamidino-2-phenylindole (DAPI). Antibodies in LAGAP-immunized C57BL/6 and BALB/cJ mice do not differ in isotype but rather in antigen specificity with only C57BL/6 serum recognizing the merozoite surface.

%I PLOS Pathogens