Pam3CSK4 induced immune tolerance against HCV NS3 mediated inflammation. Ayilam Ramachandran Rajalakshmy Jambulingam Malathi Hajib Naraharirao Madhavan 10.1371/journal.pone.0125419.g008 https://plos.figshare.com/articles/figure/_Pam3CSK4_induced_immune_tolerance_against_HCV_NS3_mediated_inflammation_/1411840 <p>Microglia was pre treated with 50 ng/ml of Pam3CSK4 for 16 hrs. RT-PCR and ELISA was performed to study the immune tolerance mediated by Pam3CSK4. <b>(A, B, C and D)</b> Pam3CSK4 treatment completely blocked IL-8, IL-6 and TNF-α gene expression and IL-1β gene expression was down regulated compared to positive control (PC NC represents the PCR reagent negative control. <b>(E, F, G and H)</b> TNF-α and IL-1β were secreted at a significant level in the Pam3CSK4 treated cells even after replacing the cells with fresh growth medium without agonist (## p< 0.01). TNF-α and IL-1β were significantly upregulated in Pam3CSK4 + NS3 treated cells compared to Pam3CSK4 exposed cells (^ p < 0.05, ^^ p < 0.01). There was a significant down regulation for IL-8 and other 3 cytokines in the Pam3CSK4 + NS3 treated cells compared to NS3 alone treated cells (** p < 0.01). The data is expressed as mean (n = 3) ± SE.</p> 2015-05-12 02:50:40 NS 3 Tumor nicrosis factor HCV NS 3 protein Receptor Ligand Treatment Furnished Immune Tolerance BackgroundRecent evidence TLR agonists Pam 3CSK hepatitis C Virus NS 3 Mediated Microglial Inflammation ligands Pam 2CSK nf Hepatitis C virus elisa Nuclear Factor kappaB microglial cell line CHME 3.MethodsIL NS 3 protein cytokine gene expression cns tnf NS 3. TLR ligand treatment facs