Overview of experimental set-up used to recellularize decellularized lung scaffolds with MSCs or C10 cells in static and bioreactor conditions.
Aurélie Crabbé
Yulong Liu
Shameema F. Sarker
Nicholas R. Bonenfant
Jennifer Barrila
Zachary D. Borg
James J. Lee
Daniel J. Weiss
Cheryl A. Nickerson
10.1371/journal.pone.0126846.g001
https://plos.figshare.com/articles/figure/_Overview_of_experimental_set_up_used_to_recellularize_decellularized_lung_scaffolds_with_MSCs_or_C10_cells_in_static_and_bioreactor_conditions_/1410033
<p>MSCs or C10 cells were introduced in the decellularized lung scaffolds through the cannulated trachea. Next, lungs were statically incubated for 4 days, regardless of the subsequent test condition. Culture medium for MSCs was IMDM and for C10 cells GTSF-2. Different time points were tested to assess recellularization with MSCs (3, 10, 24 days) or C10 cells (7, 10 days) in static or bioreactor conditions.</p>
2015-05-11 03:10:07
decellularized lungs
decellularized mouse lungs recellularized
Decellularized Lung Scaffolds
fluid shear suspension bioreactor
rwv
alveolar type II cells
msc
Dynamic Suspension Culture Strategies
mouse cell types
rna
decellularized scaffold matrix