Overview of experimental set-up used to recellularize decellularized lung scaffolds with MSCs or C10 cells in static and bioreactor conditions. Aurélie Crabbé Yulong Liu Shameema F. Sarker Nicholas R. Bonenfant Jennifer Barrila Zachary D. Borg James J. Lee Daniel J. Weiss Cheryl A. Nickerson 10.1371/journal.pone.0126846.g001 https://plos.figshare.com/articles/figure/_Overview_of_experimental_set_up_used_to_recellularize_decellularized_lung_scaffolds_with_MSCs_or_C10_cells_in_static_and_bioreactor_conditions_/1410033 <p>MSCs or C10 cells were introduced in the decellularized lung scaffolds through the cannulated trachea. Next, lungs were statically incubated for 4 days, regardless of the subsequent test condition. Culture medium for MSCs was IMDM and for C10 cells GTSF-2. Different time points were tested to assess recellularization with MSCs (3, 10, 24 days) or C10 cells (7, 10 days) in static or bioreactor conditions.</p> 2015-05-11 03:10:07 decellularized lungs decellularized mouse lungs recellularized Decellularized Lung Scaffolds fluid shear suspension bioreactor rwv alveolar type II cells msc Dynamic Suspension Culture Strategies mouse cell types rna decellularized scaffold matrix