10.1371/journal.pone.0126846.g001 Aurélie Crabbé Aurélie Crabbé Yulong Liu Yulong Liu Shameema F. Sarker Shameema F. Sarker Nicholas R. Bonenfant Nicholas R. Bonenfant Jennifer Barrila Jennifer Barrila Zachary D. Borg Zachary D. Borg James J. Lee James J. Lee Daniel J. Weiss Daniel J. Weiss Cheryl A. Nickerson Cheryl A. Nickerson Overview of experimental set-up used to recellularize decellularized lung scaffolds with MSCs or C10 cells in static and bioreactor conditions. Public Library of Science 2015 decellularized lungs decellularized mouse lungs recellularized Decellularized Lung Scaffolds fluid shear suspension bioreactor rwv alveolar type II cells msc Dynamic Suspension Culture Strategies mouse cell types rna decellularized scaffold matrix 2015-05-11 03:10:07 Figure https://plos.figshare.com/articles/figure/_Overview_of_experimental_set_up_used_to_recellularize_decellularized_lung_scaffolds_with_MSCs_or_C10_cells_in_static_and_bioreactor_conditions_/1410033 <p>MSCs or C10 cells were introduced in the decellularized lung scaffolds through the cannulated trachea. Next, lungs were statically incubated for 4 days, regardless of the subsequent test condition. Culture medium for MSCs was IMDM and for C10 cells GTSF-2. Different time points were tested to assess recellularization with MSCs (3, 10, 24 days) or C10 cells (7, 10 days) in static or bioreactor conditions.</p>