AGS-IV protects against glutamate-induced neurotoxicity in PC12 cells.
Rongcai Yue
Xia Li
Bingyang Chen
Jing Zhao
Weiwei He
Hu Yuan
Xing Yuan
Na Gao
Guozhen Wu
Huizi Jin
Lei Shan
Weidong Zhang
10.1371/journal.pone.0126603.g002
https://plos.figshare.com/articles/figure/_AGS_IV_protects_against_glutamate_induced_neurotoxicity_in_PC12_cells_/1410025
<p>(A) Dose-dependent cell death was observed after the cells were treated with different concentrations glutamate for 24 h. (B) Effects of 50 μM AGS-IV, 6-<i>O</i>-<i>β</i>-<i>D</i>-glu CAG, 3-<i>O</i>-<i>β</i>-<i>D</i>-xyl CAG, and CAG on glutamate-induced PC12 cell neurotoxicity. (C) Survival of PC12 cells after exposure to 0.1% DMSO or various concentrations of AGS-IV for 30 h. (D, E) Effects of AGS-IV on glutamate-induced PC12 cell injury. PC12 cells were treated with 10, 25, 50 and 100 μM AGS-IV and then co-incubated with or without 5 mM glutamate for 24 h, and cell cytotoxicity was determined by MTT assay and LDH activity. (F) Apoptosis assay of PC12 cells exposed to glutamate and/or AGS-IV were examined by flow cytometry. PC12 cells were gated for an annexin V<sup>+</sup> (x-axis) versus PI<sup>+</sup> (y-axis) contour plot. The numbers on dot plots represent the percentages of annexin V<sup>+</sup>/PI<sup>+</sup> cells and annexin V<sup>+</sup>/PI<sup>-</sup> cells. (G) The percentage of living cells was tested by trypan blue exclusion for PC12 cells exposed to glutamate and/or AGS-IV. All the data are presented as mean ± SEM of three independent experiments. ## < 0.01 versus vehicle and **P < 0.01 versus glutamate by one-way ANOVA analysis of variance with Tukey’s HSD post hoc test.</p>
2015-05-11 03:09:41
PC 12 cells
ppi
Chinese Materia Medica
neuroprotective effect
protein
mechanism
Astragalus membranaceus Bunge
iv
proteomic