10.1371/journal.ppat.1004763.g004
Kavi Mehta
Kavi
Mehta
Vignesh Gunasekharan
Vignesh
Gunasekharan
Ayano Satsuka
Ayano
Satsuka
Laimonis A. Laimins
Laimonis
A. Laimins
pSMC1 and pCHK2 as well as pSMC1 and γ-H2AX co-localize in distinct foci in nuclei of HPV positive cells following differentiation.
Public Library of Science
2015
sister chromatid association
insulator transcription factor CTCF
pSMC 1 forms complexes
HPV genome amplification
L 2
2AX
ATM DNA damage pathway
SMC 1
Chk 2
nbs
SMC 1 factors
ATM DNA damage response
CTCF binding motifs
Human Papillomaviruses Activate
CTCF Insulators Human papillomaviruses
Recruit SMC 1 Cohesin Proteins
shRNAs blocks genome amplification
2015-04-13 04:55:17
Figure
https://plos.figshare.com/articles/figure/_pSMC1_and_pCHK2_as_well_as_pSMC1_and_947_H2AX_co_localize_in_distinct_foci_in_nuclei_of_HPV_positive_cells_following_differentiation_/1377304
<p>CIN 612 cells were induced to differentiate by addition of high calcium media for 72 hours and examined by immunofluorescence for co-localization of A). pSMC1 and pCHK2; B), pSMC1 and γ-H2AX using the corresponding antibodies. Red signal identifies pCHK2 or γ-H2AX in top or bottom panels as shown. Green identifies pSMC1 (s957). Blue represents nuclear DAPI staining. Yellow signal in merged views demonstrates co-localization. The antibody to pCHK2 Thr68 (Cell Signalling) may be responsive to DNA damage factors in general.</p>