10.1371/journal.ppat.1004763.g004 Kavi Mehta Kavi Mehta Vignesh Gunasekharan Vignesh Gunasekharan Ayano Satsuka Ayano Satsuka Laimonis A. Laimins Laimonis A. Laimins pSMC1 and pCHK2 as well as pSMC1 and γ-H2AX co-localize in distinct foci in nuclei of HPV positive cells following differentiation. Public Library of Science 2015 sister chromatid association insulator transcription factor CTCF pSMC 1 forms complexes HPV genome amplification L 2 2AX ATM DNA damage pathway SMC 1 Chk 2 nbs SMC 1 factors ATM DNA damage response CTCF binding motifs Human Papillomaviruses Activate CTCF Insulators Human papillomaviruses Recruit SMC 1 Cohesin Proteins shRNAs blocks genome amplification 2015-04-13 04:55:17 Figure https://plos.figshare.com/articles/figure/_pSMC1_and_pCHK2_as_well_as_pSMC1_and_947_H2AX_co_localize_in_distinct_foci_in_nuclei_of_HPV_positive_cells_following_differentiation_/1377304 <p>CIN 612 cells were induced to differentiate by addition of high calcium media for 72 hours and examined by immunofluorescence for co-localization of A). pSMC1 and pCHK2; B), pSMC1 and γ-H2AX using the corresponding antibodies. Red signal identifies pCHK2 or γ-H2AX in top or bottom panels as shown. Green identifies pSMC1 (s957). Blue represents nuclear DAPI staining. Yellow signal in merged views demonstrates co-localization. The antibody to pCHK2 Thr68 (Cell Signalling) may be responsive to DNA damage factors in general.</p>