Cryo-EM analysis of plant-produced CoHe-GFPL VLPs. Hadrien Peyret Annick Gehin Eva C. Thuenemann Donatienne Blond Aadil El Turabi Lucy Beales Dean Clarke Robert J. C. Gilbert Elizabeth E. Fry David I. Stuart Kris Holmes Nicola J. Stonehouse Mike Whelan William Rosenberg George P. Lomonossoff David J. Rowlands 10.1371/journal.pone.0120751.g006 https://plos.figshare.com/articles/figure/_Cryo_EM_analysis_of_plant_produced_CoHe_GFPL_VLPs_/1364123 <p>a) Particles were flash-frozen in vitreous ice, then subjected to cryo-electron microscopy. Class averages were obtained from 441 individual particles using EMAN software. The expanded view (lower right) is of an average of all images used. b) 3D reconstruction of the particles using icosahedral symmetry, superimposed on the He map as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120751#pone.0120751.g003" target="_blank">Fig. 3</a>. The CoHe-GFPL map is coloured red-to-blue from the centre of the volume towards its edge; the He map is shown in grey.</p> 2015-04-01 03:12:01 HBc particles Hepatitis B Core Antigen Allows Assembly mir Hepatitis B virus hbcag sequence nanobody vlp Accommodate Foreign Proteins tandem core technology surface spike structures tandem core particles tandem core particle insertion protein gfp