Cryo-EM analysis of plant-produced CoHe-GFPL VLPs.
Hadrien Peyret
Annick Gehin
Eva C. Thuenemann
Donatienne Blond
Aadil El Turabi
Lucy Beales
Dean Clarke
Robert J. C. Gilbert
Elizabeth E. Fry
David I. Stuart
Kris Holmes
Nicola J. Stonehouse
Mike Whelan
William Rosenberg
George P. Lomonossoff
David J. Rowlands
10.1371/journal.pone.0120751.g006
https://plos.figshare.com/articles/figure/_Cryo_EM_analysis_of_plant_produced_CoHe_GFPL_VLPs_/1364123
<p>a) Particles were flash-frozen in vitreous ice, then subjected to cryo-electron microscopy. Class averages were obtained from 441 individual particles using EMAN software. The expanded view (lower right) is of an average of all images used. b) 3D reconstruction of the particles using icosahedral symmetry, superimposed on the He map as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120751#pone.0120751.g003" target="_blank">Fig. 3</a>. The CoHe-GFPL map is coloured red-to-blue from the centre of the volume towards its edge; the He map is shown in grey.</p>
2015-04-01 03:12:01
HBc particles
Hepatitis B Core Antigen Allows Assembly
mir
Hepatitis B virus
hbcag
sequence
nanobody
vlp
Accommodate Foreign Proteins
tandem core technology
surface spike structures
tandem core particles
tandem core particle
insertion
protein
gfp