10.1371/journal.pone.0120751.g006 Hadrien Peyret Hadrien Peyret Annick Gehin Annick Gehin Eva C. Thuenemann Eva C. Thuenemann Donatienne Blond Donatienne Blond Aadil El Turabi Aadil El Turabi Lucy Beales Lucy Beales Dean Clarke Dean Clarke Robert J. C. Gilbert Robert J. C. Gilbert Elizabeth E. Fry Elizabeth E. Fry David I. Stuart David I. Stuart Kris Holmes Kris Holmes Nicola J. Stonehouse Nicola J. Stonehouse Mike Whelan Mike Whelan William Rosenberg William Rosenberg George P. Lomonossoff George P. Lomonossoff David J. Rowlands David J. Rowlands Cryo-EM analysis of plant-produced CoHe-GFPL VLPs. Public Library of Science 2015 HBc particles Hepatitis B Core Antigen Allows Assembly mir Hepatitis B virus hbcag sequence nanobody vlp Accommodate Foreign Proteins tandem core technology surface spike structures tandem core particles tandem core particle insertion protein gfp 2015-04-01 03:12:01 Figure https://plos.figshare.com/articles/figure/_Cryo_EM_analysis_of_plant_produced_CoHe_GFPL_VLPs_/1364123 <p>a) Particles were flash-frozen in vitreous ice, then subjected to cryo-electron microscopy. Class averages were obtained from 441 individual particles using EMAN software. The expanded view (lower right) is of an average of all images used. b) 3D reconstruction of the particles using icosahedral symmetry, superimposed on the He map as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120751#pone.0120751.g003" target="_blank">Fig. 3</a>. The CoHe-GFPL map is coloured red-to-blue from the centre of the volume towards its edge; the He map is shown in grey.</p>