%0 Figure %A Peyret, Hadrien %A Gehin, Annick %A C. Thuenemann, Eva %A Blond, Donatienne %A El Turabi, Aadil %A Beales, Lucy %A Clarke, Dean %A J. C. Gilbert, Robert %A E. Fry, Elizabeth %A Stuart, David I. %A Holmes, Kris %A J. Stonehouse, Nicola %A Whelan, Mike %A Rosenberg, William %A Lomonossoff, George P. %A J. Rowlands, David %D 2015 %T Cryo-electron microscopy analysis of E. coli- produced tandem core particles. %U https://plos.figshare.com/articles/figure/_Cryo_electron_microscopy_analysis_of_E_coli_produced_tandem_core_particles_/1364120 %R 10.1371/journal.pone.0120751.g003 %2 https://plos.figshare.com/ndownloader/files/2001268 %K HBc particles %K Hepatitis B Core Antigen Allows Assembly %K mir %K Hepatitis B virus %K hbcag %K sequence %K nanobody %K vlp %K Accommodate Foreign Proteins %K tandem core technology %K surface spike structures %K tandem core particles %K tandem core particle %K insertion %K protein %K gfp %X

a) Surface-rendered views of the reconstructions. Red—hetero-tandem core, contoured at 1σ. Green—homo-tandem core, contoured at 1σ. Blue—difference map, hetero-minus-homo, contoured at 4σ. b) Transverse view across 5-fold axis of the He core with co-ordinates from the HBc crystal structure (Wynne et al., 1999) fitted into the EM density. c) Density profiles of the He (red) and Ho (green) cores generated from translationally-aligned rotational averages. For comparison central sections of the He (upper panel) and Ho (lower panel) maps are shown to the right. A ring of density under the main capsid surface and at a radius of ~90 Å derives from the protamine-like region in He.

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