%0 Figure %A Siddappa, Dayananda %A Beaulieu, Élaine %A Gévry, Nicolas %A P. Roux, Philippe %A Bordignon, Vilceu %A Duggavathi, Raj %D 2015 %T Inhibition of Mapk3/1 activity and knockdown of Egr1 results in reduced induction of Ptgs2 in primary granulosa cells in vitro. %U https://plos.figshare.com/articles/figure/_Inhibition_of_Mapk3_1_activity_and_knockdown_of_Egr1_results_in_reduced_induction_of_Ptgs2_in_primary_granulosa_cells_in_vitro_/1353645 %R 10.1371/journal.pone.0119387.g007 %2 https://plos.figshare.com/ndownloader/files/1985907 %K Ptgs 2 promoter %K EGR 1 %K granulosa cells %K Egr 1 antibody %K pd %K GRMO %K ovulation %K gene %K PTGS 2 %K mapk %K growth response 1 %K EGR 1 expression %K LH signal transduction %K Transient Pharmacological Inhibition %K Egr 1 transcript accumulation %X

A) Treatment with PD0325901 decreased Fo+PMA induced increases in the abundance of Ptgs2 protein relative to vehicle treated primary granulosa cells. B) Pre-treatment with Egr1-siRNA inhibited Fo+PMA induced expression of Egr1 when compared to control siRNA treatment in primary granulosa cells in vitro. This was associated with reduced abundance of Pgts2 transcript in Egr1-siRNA treated cells. Fo—forskolin; PMA—phorbol-12-myristate.

%I PLOS ONE