10.1371/journal.pgen.1004913
Sebastian Treusch
Sebastian
Treusch
Frank W. Albert
Frank
W. Albert
Joshua S. Bloom
Joshua S.
Bloom
Iulia E. Kotenko
Iulia
E. Kotenko
Leonid Kruglyak
Leonid
Kruglyak
Genetic Mapping of MAPK-Mediated Complex Traits Across <i>S. cerevisiae</i>
Public Library of Science
2015
genetic mapping
search space
Trait locus
strain background
mapping results
mapping approach
phenotypic differences
mapk
genetic variants
cerevisiae Signaling pathways
phenotypic consequences
phenotypic diversity
influence phenotypes
QTL fall
2015-01-08 03:29:57
Dataset
https://plos.figshare.com/articles/dataset/Genetic_Mapping_of_MAPK_Mediated_Complex_Traits_Across_S_cerevisiae_/1286637
<div><p>Signaling pathways enable cells to sense and respond to their environment. Many cellular signaling strategies are conserved from fungi to humans, yet their activity and phenotypic consequences can vary extensively among individuals within a species. A systematic assessment of the impact of naturally occurring genetic variation on signaling pathways remains to be conducted. In <i>S. cerevisiae</i>, both response and resistance to stressors that activate signaling pathways differ between diverse isolates. Here, we present a quantitative trait locus (QTL) mapping approach that enables us to identify genetic variants underlying such phenotypic differences across the genetic and phenotypic diversity of <i>S. cerevisiae</i>. Using a Round-robin cross between twelve diverse strains, we identified QTL that influence phenotypes critically dependent on MAPK signaling cascades. Genetic variants under these QTL fall within MAPK signaling networks themselves as well as other interconnected signaling pathways. Finally, we demonstrate how the mapping results from multiple strain background can be leveraged to narrow the search space of causal genetic variants.</p></div>