The VZV ORF20 RHIM forms heteromeric amyloid fibrils with ZBP1, which are dependent on the core tetrad for stable higher-order assembly.
Megan Steain
Max O. D. G. Baker
Chi L. L. Pham
Nirukshan Shanmugam
Yann Gambin
Emma Sierecki
Brian P. McSharry
Selmir Avdic
Barry Slobedman
Margaret Sunde
Allison Abendroth
10.1371/journal.ppat.1008473.g009
https://plos.figshare.com/articles/figure/The_VZV_ORF20_RHIM_forms_heteromeric_amyloid_fibrils_with_ZBP1_which_are_dependent_on_the_core_tetrad_for_stable_higher-order_assembly_/12642483
<p>(A) Transmission electron micrograph of protein assemblies formed from combinations of Ub-ORF20<sub>1-114</sub>, Ub-ORF20<sub>1-114</sub>mut, and YPet-ZBP1<sub>170-355</sub> fusion proteins. Scale bar represents 200 nm. (B) Confocal microscope images of heterofibrils formed from a mixture of ORF20<sub>1-114</sub>-mCherry and YPet-ZBP1<sub>170-355</sub> in ThT-containing assembly buffer. Scale bar represents 20 μm. (C) The IQIG core tetrad from ORF20 is required to trap ZBP1 in insoluble, detergent-stable aggregates. SDS-AGE analysis of monomeric or assembled forms of Ub-ORF20<sub>1-114</sub>, Ub-ORF20<sub>1-114</sub>mut, and YPetZBP1<sub>170-355</sub>, or combinations thereof. Monomeric proteins were maintained in 8 M urea before electrophoresis. Assembled samples were incubated with either water or 2% SDS at room temperature for 10 min before electrophoresis. Identity and treatment of protein indicated above each sample.</p>
2020-07-10 17:39:37
Varicella zoster virus
host cell death
ZBP 1-driven apoptosis
VZV infection
VZV capsid triplex protein
amyloid complexes
TNF-induced necroptosis
decoy amyloid assemblies
ZBP 1 pathway
sequestering ZBP 1
RIP Homotypic Interaction Motif
host cell death inhibitor
VZV ORF 20 RHIM
Varicella zoster virus encodes
RIPK
cell death Herpesviruses
ORF 20 RHIM
ORF 20 RHIM forms
ZBP 1-expressing HT -29 cells