Steain, Megan Baker, Max O. D. G. Pham, Chi L. L. Shanmugam, Nirukshan Gambin, Yann Sierecki, Emma McSharry, Brian P. Avdic, Selmir Slobedman, Barry Sunde, Margaret Abendroth, Allison The VZV ORF20 RHIM forms heteromeric complexes with RHIMs from RIPK3 and ZBP1. <p>(A) Schematic representation of fluorescence detection from dilute solutions containing two different fluorophores in a nanolitre confocal volume. Samples are excited simultaneously with two overlapping lasers and emission from the YPet and mCherry fluorophores is recorded separately. Coincident bursts in the two channels indicate formation of heteromeric complexes containing two different proteins (B-I). Representative fluorescent time traces collected from ORF20<sub>1-114</sub>-mCherry, ORF20<sub>1-114</sub>mut-mCherry, YPet-RIPK3<sub>387-518</sub> or YPet-ZBP1<sub>170-355</sub> fusion proteins, alone or mixed in pairs under conditions that allow co-assembly. The proteins present in each mixture are indicated for each part of the figure. Inserts show detail of 1 s dual fluorescence recording, indicated by star on the full time trace.</p> Varicella zoster virus;host cell death;ZBP 1-driven apoptosis;VZV infection;VZV capsid triplex protein;amyloid complexes;TNF-induced necroptosis;decoy amyloid assemblies;ZBP 1 pathway;sequestering ZBP 1;RIP Homotypic Interaction Motif;host cell death inhibitor;VZV ORF 20 RHIM;Varicella zoster virus encodes;RIPK;cell death Herpesviruses;ORF 20 RHIM;ORF 20 RHIM forms;ZBP 1-expressing HT -29 cells 2020-07-10
    https://plos.figshare.com/articles/figure/The_VZV_ORF20_RHIM_forms_heteromeric_complexes_with_RHIMs_from_RIPK3_and_ZBP1_/12642480
10.1371/journal.ppat.1008473.g008