10.1371/journal.ppat.1008473.g008 Megan Steain Megan Steain Max O. D. G. Baker Max O. D. G. Baker Chi L. L. Pham Chi L. L. Pham Nirukshan Shanmugam Nirukshan Shanmugam Yann Gambin Yann Gambin Emma Sierecki Emma Sierecki Brian P. McSharry Brian P. McSharry Selmir Avdic Selmir Avdic Barry Slobedman Barry Slobedman Margaret Sunde Margaret Sunde Allison Abendroth Allison Abendroth The VZV ORF20 RHIM forms heteromeric complexes with RHIMs from RIPK3 and ZBP1. Public Library of Science 2020 Varicella zoster virus host cell death ZBP 1-driven apoptosis VZV infection VZV capsid triplex protein amyloid complexes TNF-induced necroptosis decoy amyloid assemblies ZBP 1 pathway sequestering ZBP 1 RIP Homotypic Interaction Motif host cell death inhibitor VZV ORF 20 RHIM Varicella zoster virus encodes RIPK cell death Herpesviruses ORF 20 RHIM ORF 20 RHIM forms ZBP 1-expressing HT -29 cells 2020-07-10 17:39:36 Figure https://plos.figshare.com/articles/figure/The_VZV_ORF20_RHIM_forms_heteromeric_complexes_with_RHIMs_from_RIPK3_and_ZBP1_/12642480 <p>(A) Schematic representation of fluorescence detection from dilute solutions containing two different fluorophores in a nanolitre confocal volume. Samples are excited simultaneously with two overlapping lasers and emission from the YPet and mCherry fluorophores is recorded separately. Coincident bursts in the two channels indicate formation of heteromeric complexes containing two different proteins (B-I). Representative fluorescent time traces collected from ORF20<sub>1-114</sub>-mCherry, ORF20<sub>1-114</sub>mut-mCherry, YPet-RIPK3<sub>387-518</sub> or YPet-ZBP1<sub>170-355</sub> fusion proteins, alone or mixed in pairs under conditions that allow co-assembly. The proteins present in each mixture are indicated for each part of the figure. Inserts show detail of 1 s dual fluorescence recording, indicated by star on the full time trace.</p>