10.1371/journal.ppat.1008473.g008
Megan Steain
Megan
Steain
Max O. D. G. Baker
Max O. D. G.
Baker
Chi L. L. Pham
Chi L. L.
Pham
Nirukshan Shanmugam
Nirukshan
Shanmugam
Yann Gambin
Yann
Gambin
Emma Sierecki
Emma
Sierecki
Brian P. McSharry
Brian P.
McSharry
Selmir Avdic
Selmir
Avdic
Barry Slobedman
Barry
Slobedman
Margaret Sunde
Margaret
Sunde
Allison Abendroth
Allison
Abendroth
The VZV ORF20 RHIM forms heteromeric complexes with RHIMs from RIPK3 and ZBP1.
Public Library of Science
2020
Varicella zoster virus
host cell death
ZBP 1-driven apoptosis
VZV infection
VZV capsid triplex protein
amyloid complexes
TNF-induced necroptosis
decoy amyloid assemblies
ZBP 1 pathway
sequestering ZBP 1
RIP Homotypic Interaction Motif
host cell death inhibitor
VZV ORF 20 RHIM
Varicella zoster virus encodes
RIPK
cell death Herpesviruses
ORF 20 RHIM
ORF 20 RHIM forms
ZBP 1-expressing HT -29 cells
2020-07-10 17:39:36
Figure
https://plos.figshare.com/articles/figure/The_VZV_ORF20_RHIM_forms_heteromeric_complexes_with_RHIMs_from_RIPK3_and_ZBP1_/12642480
<p>(A) Schematic representation of fluorescence detection from dilute solutions containing two different fluorophores in a nanolitre confocal volume. Samples are excited simultaneously with two overlapping lasers and emission from the YPet and mCherry fluorophores is recorded separately. Coincident bursts in the two channels indicate formation of heteromeric complexes containing two different proteins (B-I). Representative fluorescent time traces collected from ORF20<sub>1-114</sub>-mCherry, ORF20<sub>1-114</sub>mut-mCherry, YPet-RIPK3<sub>387-518</sub> or YPet-ZBP1<sub>170-355</sub> fusion proteins, alone or mixed in pairs under conditions that allow co-assembly. The proteins present in each mixture are indicated for each part of the figure. Inserts show detail of 1 s dual fluorescence recording, indicated by star on the full time trace.</p>