%0 Figure %A Steain, Megan %A Baker, Max O. D. G. %A Pham, Chi L. L. %A Shanmugam, Nirukshan %A Gambin, Yann %A Sierecki, Emma %A McSharry, Brian P. %A Avdic, Selmir %A Slobedman, Barry %A Sunde, Margaret %A Abendroth, Allison %D 2020 %T The ORF20 RHIM forms homomeric amyloid structures. %U https://plos.figshare.com/articles/figure/The_ORF20_RHIM_forms_homomeric_amyloid_structures_/12642477 %R 10.1371/journal.ppat.1008473.g007 %2 https://plos.figshare.com/ndownloader/files/23775111 %K Varicella zoster virus %K host cell death %K ZBP 1-driven apoptosis %K VZV infection %K VZV capsid triplex protein %K amyloid complexes %K TNF-induced necroptosis %K decoy amyloid assemblies %K ZBP 1 pathway %K sequestering ZBP 1 %K RIP Homotypic Interaction Motif %K host cell death inhibitor %K VZV ORF 20 RHIM %K Varicella zoster virus encodes %K RIPK %K cell death Herpesviruses %K ORF 20 RHIM %K ORF 20 RHIM forms %K ZBP 1-expressing HT -29 cells %X

(A) Transmission electron microscopy image of structures formed by Ub-ORF201-114. Scale bar represents 200 nm. (B) Transmission electron microscopy image of structures formed by mCherry-ORF201-114. Scale bar represents 200 nm. (C) ThT fluorescence over time of Ub-ORF20 constructs after dilution from 8 M urea into assembly buffer. Buffer sample contains equimolar ThT but no protein. Curves are derived from three independent replicates. Error bars indicate standard deviation. (D) Absorbance spectra of solutions containing Congo red and Ub-ORF201-114 and Ub-ORF201-114mut after dialysis against assembly buffer. Buffer refers to a Congo red sample in assembly buffer with no protein. (E) Transmission electron microscopy image of structures formed by Ub-ORF201-114mut. Scale bar represents 200 nm. (F) Transmission electron microscopy image of structures formed by mCherry-ORF201-114mut. Scale bar represents 200 nm.

%I PLOS Pathogens