The ORF20 RHIM forms homomeric amyloid structures.
Megan Steain
Max O. D. G. Baker
Chi L. L. Pham
Nirukshan Shanmugam
Yann Gambin
Emma Sierecki
Brian P. McSharry
Selmir Avdic
Barry Slobedman
Margaret Sunde
Allison Abendroth
10.1371/journal.ppat.1008473.g007
https://plos.figshare.com/articles/figure/The_ORF20_RHIM_forms_homomeric_amyloid_structures_/12642477
<p>(A) Transmission electron microscopy image of structures formed by Ub-ORF20<sub>1-114</sub>. Scale bar represents 200 nm. (B) Transmission electron microscopy image of structures formed by mCherry-ORF20<sub>1-114</sub>. Scale bar represents 200 nm. (C) ThT fluorescence over time of Ub-ORF20 constructs after dilution from 8 M urea into assembly buffer. Buffer sample contains equimolar ThT but no protein. Curves are derived from three independent replicates. Error bars indicate standard deviation. (D) Absorbance spectra of solutions containing Congo red and Ub-ORF20<sub>1-114</sub> and Ub-ORF20<sub>1-114</sub>mut after dialysis against assembly buffer. Buffer refers to a Congo red sample in assembly buffer with no protein. (E) Transmission electron microscopy image of structures formed by Ub-ORF20<sub>1-114</sub>mut. Scale bar represents 200 nm. (F) Transmission electron microscopy image of structures formed by mCherry-ORF20<sub>1-114</sub>mut. Scale bar represents 200 nm.</p>
2020-07-10 17:39:34
Varicella zoster virus
host cell death
ZBP 1-driven apoptosis
VZV infection
VZV capsid triplex protein
amyloid complexes
TNF-induced necroptosis
decoy amyloid assemblies
ZBP 1 pathway
sequestering ZBP 1
RIP Homotypic Interaction Motif
host cell death inhibitor
VZV ORF 20 RHIM
Varicella zoster virus encodes
RIPK
cell death Herpesviruses
ORF 20 RHIM
ORF 20 RHIM forms
ZBP 1-expressing HT -29 cells