Steain, Megan Baker, Max O. D. G. Pham, Chi L. L. Shanmugam, Nirukshan Gambin, Yann Sierecki, Emma McSharry, Brian P. Avdic, Selmir Slobedman, Barry Sunde, Margaret Abendroth, Allison ORF20 interacts with human RHIM proteins associated with cell death signalling. <p>293T cells were transfected with ORF20 constructs and ZBP1-GFP or GFP alone. Cells were harvested and immunoprecipitation (IP) performed on the soluble and insoluble fractions using GFP as bait. Western blot (IB) was performed on the immunoprecipitated and input cell lysates. (A) Immunoprecipitation of ZBP1-GFP in the soluble fraction of cell lysates. (B) Immunoprecipitation of ZBP1-GFP in the insoluble fraction of cell lysates. (C) Immunoprecipitation of RIPK3-GFP in the soluble fraction of cell lysates. Each blot is representative of at least 2 independent biological replicates. Arrows indicate protein size markers.</p> Varicella zoster virus;host cell death;ZBP 1-driven apoptosis;VZV infection;VZV capsid triplex protein;amyloid complexes;TNF-induced necroptosis;decoy amyloid assemblies;ZBP 1 pathway;sequestering ZBP 1;RIP Homotypic Interaction Motif;host cell death inhibitor;VZV ORF 20 RHIM;Varicella zoster virus encodes;RIPK;cell death Herpesviruses;ORF 20 RHIM;ORF 20 RHIM forms;ZBP 1-expressing HT -29 cells 2020-07-10
    https://plos.figshare.com/articles/figure/ORF20_interacts_with_human_RHIM_proteins_associated_with_cell_death_signalling_/12642474
10.1371/journal.ppat.1008473.g006