10.1371/journal.ppat.1008473.g006 Megan Steain Megan Steain Max O. D. G. Baker Max O. D. G. Baker Chi L. L. Pham Chi L. L. Pham Nirukshan Shanmugam Nirukshan Shanmugam Yann Gambin Yann Gambin Emma Sierecki Emma Sierecki Brian P. McSharry Brian P. McSharry Selmir Avdic Selmir Avdic Barry Slobedman Barry Slobedman Margaret Sunde Margaret Sunde Allison Abendroth Allison Abendroth ORF20 interacts with human RHIM proteins associated with cell death signalling. Public Library of Science 2020 Varicella zoster virus host cell death ZBP 1-driven apoptosis VZV infection VZV capsid triplex protein amyloid complexes TNF-induced necroptosis decoy amyloid assemblies ZBP 1 pathway sequestering ZBP 1 RIP Homotypic Interaction Motif host cell death inhibitor VZV ORF 20 RHIM Varicella zoster virus encodes RIPK cell death Herpesviruses ORF 20 RHIM ORF 20 RHIM forms ZBP 1-expressing HT -29 cells 2020-07-10 17:39:33 Figure https://plos.figshare.com/articles/figure/ORF20_interacts_with_human_RHIM_proteins_associated_with_cell_death_signalling_/12642474 <p>293T cells were transfected with ORF20 constructs and ZBP1-GFP or GFP alone. Cells were harvested and immunoprecipitation (IP) performed on the soluble and insoluble fractions using GFP as bait. Western blot (IB) was performed on the immunoprecipitated and input cell lysates. (A) Immunoprecipitation of ZBP1-GFP in the soluble fraction of cell lysates. (B) Immunoprecipitation of ZBP1-GFP in the insoluble fraction of cell lysates. (C) Immunoprecipitation of RIPK3-GFP in the soluble fraction of cell lysates. Each blot is representative of at least 2 independent biological replicates. Arrows indicate protein size markers.</p>