ZBP1 restricts VZV-RHIMmut and VZV-RHIMKO spread in culture. Megan Steain Max O. D. G. Baker Chi L. L. Pham Nirukshan Shanmugam Yann Gambin Emma Sierecki Brian P. McSharry Selmir Avdic Barry Slobedman Margaret Sunde Allison Abendroth 10.1371/journal.ppat.1008473.g004 https://plos.figshare.com/articles/figure/ZBP1_restricts_VZV-RHIMmut_and_VZV-RHIMKO_spread_in_culture_/12642468 <p>Empty vector or ZBP1 expressing HT-29s were inoculated with parental HT-29 cells either mock or infected with parent VZV (pOKA), VZV-RHIMmut or VZV-RHIMKO. z-VAD–fmk (25 μM) or necrosulfonamide (NSA; 1 μM) was added at the time of inoculation where indicated. (A) After 72 h cells were fixed and immunostained for VZV IE63 (red) to assess virus spread. Images are representative from at least 2 independent replicates. Scale bar indicates 500 μm. (B) The area of 18–20 plaques per virus (as indicated) was calculated using Zen 3.1 Blue edition (Zeiss), and statistical significance calculated using a one-way ANOVA. Line indicates mean and error bars represent standard error of the mean.</p> 2020-07-10 17:39:31 Varicella zoster virus host cell death ZBP 1-driven apoptosis VZV infection VZV capsid triplex protein amyloid complexes TNF-induced necroptosis decoy amyloid assemblies ZBP 1 pathway sequestering ZBP 1 RIP Homotypic Interaction Motif host cell death inhibitor VZV ORF 20 RHIM Varicella zoster virus encodes RIPK cell death Herpesviruses ORF 20 RHIM ORF 20 RHIM forms ZBP 1-expressing HT -29 cells