ZBP1 restricts VZV-RHIMmut and VZV-RHIMKO spread in culture.
Megan Steain
Max O. D. G. Baker
Chi L. L. Pham
Nirukshan Shanmugam
Yann Gambin
Emma Sierecki
Brian P. McSharry
Selmir Avdic
Barry Slobedman
Margaret Sunde
Allison Abendroth
10.1371/journal.ppat.1008473.g004
https://plos.figshare.com/articles/figure/ZBP1_restricts_VZV-RHIMmut_and_VZV-RHIMKO_spread_in_culture_/12642468
<p>Empty vector or ZBP1 expressing HT-29s were inoculated with parental HT-29 cells either mock or infected with parent VZV (pOKA), VZV-RHIMmut or VZV-RHIMKO. z-VAD–fmk (25 μM) or necrosulfonamide (NSA; 1 μM) was added at the time of inoculation where indicated. (A) After 72 h cells were fixed and immunostained for VZV IE63 (red) to assess virus spread. Images are representative from at least 2 independent replicates. Scale bar indicates 500 μm. (B) The area of 18–20 plaques per virus (as indicated) was calculated using Zen 3.1 Blue edition (Zeiss), and statistical significance calculated using a one-way ANOVA. Line indicates mean and error bars represent standard error of the mean.</p>
2020-07-10 17:39:31
Varicella zoster virus
host cell death
ZBP 1-driven apoptosis
VZV infection
VZV capsid triplex protein
amyloid complexes
TNF-induced necroptosis
decoy amyloid assemblies
ZBP 1 pathway
sequestering ZBP 1
RIP Homotypic Interaction Motif
host cell death inhibitor
VZV ORF 20 RHIM
Varicella zoster virus encodes
RIPK
cell death Herpesviruses
ORF 20 RHIM
ORF 20 RHIM forms
ZBP 1-expressing HT -29 cells