Steain, Megan Baker, Max O. D. G. Pham, Chi L. L. Shanmugam, Nirukshan Gambin, Yann Sierecki, Emma McSharry, Brian P. Avdic, Selmir Slobedman, Barry Sunde, Margaret Abendroth, Allison The VZV ORF20 RHIM is not involved in TNF-induced necroptosis. <p>(A) Viability of mock, VZV and VZV RHIM mutated (VZV-RHIMmut) virus infected HT-29s (72 h post-infection) following treatments with TNF (T; 30 ng/ml), BV-6 (S; 1 μM), z-VAD-fmk (V; 25 μM) and necrostatin-1 (Nec1; 30 μM) alone or in combination as indicated. Data was normalised to DMSO only control. Error bars show standard error of the mean, from 4 independent replicates and statistical significance was determined using a two-way ANOVA.</p> Varicella zoster virus;host cell death;ZBP 1-driven apoptosis;VZV infection;VZV capsid triplex protein;amyloid complexes;TNF-induced necroptosis;decoy amyloid assemblies;ZBP 1 pathway;sequestering ZBP 1;RIP Homotypic Interaction Motif;host cell death inhibitor;VZV ORF 20 RHIM;Varicella zoster virus encodes;RIPK;cell death Herpesviruses;ORF 20 RHIM;ORF 20 RHIM forms;ZBP 1-expressing HT -29 cells 2020-07-10
    https://plos.figshare.com/articles/figure/The_VZV_ORF20_RHIM_is_not_involved_in_TNF-induced_necroptosis_/12642462
10.1371/journal.ppat.1008473.g003