10.1371/journal.ppat.1008473.g003 Megan Steain Megan Steain Max O. D. G. Baker Max O. D. G. Baker Chi L. L. Pham Chi L. L. Pham Nirukshan Shanmugam Nirukshan Shanmugam Yann Gambin Yann Gambin Emma Sierecki Emma Sierecki Brian P. McSharry Brian P. McSharry Selmir Avdic Selmir Avdic Barry Slobedman Barry Slobedman Margaret Sunde Margaret Sunde Allison Abendroth Allison Abendroth The VZV ORF20 RHIM is not involved in TNF-induced necroptosis. Public Library of Science 2020 Varicella zoster virus host cell death ZBP 1-driven apoptosis VZV infection VZV capsid triplex protein amyloid complexes TNF-induced necroptosis decoy amyloid assemblies ZBP 1 pathway sequestering ZBP 1 RIP Homotypic Interaction Motif host cell death inhibitor VZV ORF 20 RHIM Varicella zoster virus encodes RIPK cell death Herpesviruses ORF 20 RHIM ORF 20 RHIM forms ZBP 1-expressing HT -29 cells 2020-07-10 17:39:29 Figure https://plos.figshare.com/articles/figure/The_VZV_ORF20_RHIM_is_not_involved_in_TNF-induced_necroptosis_/12642462 <p>(A) Viability of mock, VZV and VZV RHIM mutated (VZV-RHIMmut) virus infected HT-29s (72 h post-infection) following treatments with TNF (T; 30 ng/ml), BV-6 (S; 1 μM), z-VAD-fmk (V; 25 μM) and necrostatin-1 (Nec1; 30 μM) alone or in combination as indicated. Data was normalised to DMSO only control. Error bars show standard error of the mean, from 4 independent replicates and statistical significance was determined using a two-way ANOVA.</p>