10.1371/journal.ppat.1008473.g003
Megan Steain
Megan
Steain
Max O. D. G. Baker
Max O. D. G.
Baker
Chi L. L. Pham
Chi L. L.
Pham
Nirukshan Shanmugam
Nirukshan
Shanmugam
Yann Gambin
Yann
Gambin
Emma Sierecki
Emma
Sierecki
Brian P. McSharry
Brian P.
McSharry
Selmir Avdic
Selmir
Avdic
Barry Slobedman
Barry
Slobedman
Margaret Sunde
Margaret
Sunde
Allison Abendroth
Allison
Abendroth
The VZV ORF20 RHIM is not involved in TNF-induced necroptosis.
Public Library of Science
2020
Varicella zoster virus
host cell death
ZBP 1-driven apoptosis
VZV infection
VZV capsid triplex protein
amyloid complexes
TNF-induced necroptosis
decoy amyloid assemblies
ZBP 1 pathway
sequestering ZBP 1
RIP Homotypic Interaction Motif
host cell death inhibitor
VZV ORF 20 RHIM
Varicella zoster virus encodes
RIPK
cell death Herpesviruses
ORF 20 RHIM
ORF 20 RHIM forms
ZBP 1-expressing HT -29 cells
2020-07-10 17:39:29
Figure
https://plos.figshare.com/articles/figure/The_VZV_ORF20_RHIM_is_not_involved_in_TNF-induced_necroptosis_/12642462
<p>(A) Viability of mock, VZV and VZV RHIM mutated (VZV-RHIMmut) virus infected HT-29s (72 h post-infection) following treatments with TNF (T; 30 ng/ml), BV-6 (S; 1 μM), z-VAD-fmk (V; 25 μM) and necrostatin-1 (Nec1; 30 μM) alone or in combination as indicated. Data was normalised to DMSO only control. Error bars show standard error of the mean, from 4 independent replicates and statistical significance was determined using a two-way ANOVA.</p>