10.1371/journal.ppat.1008473.g002
Megan Steain
Megan
Steain
Max O. D. G. Baker
Max O. D. G.
Baker
Chi L. L. Pham
Chi L. L.
Pham
Nirukshan Shanmugam
Nirukshan
Shanmugam
Yann Gambin
Yann
Gambin
Emma Sierecki
Emma
Sierecki
Brian P. McSharry
Brian P.
McSharry
Selmir Avdic
Selmir
Avdic
Barry Slobedman
Barry
Slobedman
Margaret Sunde
Margaret
Sunde
Allison Abendroth
Allison
Abendroth
VZV infection inhibits phosphorylation of MLKL during TNF-induced necroptosis.
Public Library of Science
2020
Varicella zoster virus
host cell death
ZBP 1-driven apoptosis
VZV infection
VZV capsid triplex protein
amyloid complexes
TNF-induced necroptosis
decoy amyloid assemblies
ZBP 1 pathway
sequestering ZBP 1
RIP Homotypic Interaction Motif
host cell death inhibitor
VZV ORF 20 RHIM
Varicella zoster virus encodes
RIPK
cell death Herpesviruses
ORF 20 RHIM
ORF 20 RHIM forms
ZBP 1-expressing HT -29 cells
2020-07-10 17:39:28
Figure
https://plos.figshare.com/articles/figure/VZV_infection_inhibits_phosphorylation_of_MLKL_during_TNF-induced_necroptosis_/12642459
<p>(A) Immunofluorescence staining for phosphorylated MLKL (red) and VZV IE62 antigen (green) in mock and VZV infected HT-29 adenocarcinoma cells untreated (DMSO control) or treated with TNF (T; 30 ng/ml), BV-6 (S; 1 μM) and z-VAD-fmk (V; 25 μM) for 7–8 h to induce necroptosis. Following immunostaining cells were counterstained with DAPI (blue). (B) The percentage of cells that were pMLKL positive was determined by randomly imaging 10–20 non-overlapping regions of each slide and manually counting cells from 3 independent experiments. Error bars show standard error of the mean, statistical significance was determined using a one-way ANOVA. Scale bar indicates 20 μm.</p>