10.1371/journal.ppat.1008473.g002 Megan Steain Megan Steain Max O. D. G. Baker Max O. D. G. Baker Chi L. L. Pham Chi L. L. Pham Nirukshan Shanmugam Nirukshan Shanmugam Yann Gambin Yann Gambin Emma Sierecki Emma Sierecki Brian P. McSharry Brian P. McSharry Selmir Avdic Selmir Avdic Barry Slobedman Barry Slobedman Margaret Sunde Margaret Sunde Allison Abendroth Allison Abendroth VZV infection inhibits phosphorylation of MLKL during TNF-induced necroptosis. Public Library of Science 2020 Varicella zoster virus host cell death ZBP 1-driven apoptosis VZV infection VZV capsid triplex protein amyloid complexes TNF-induced necroptosis decoy amyloid assemblies ZBP 1 pathway sequestering ZBP 1 RIP Homotypic Interaction Motif host cell death inhibitor VZV ORF 20 RHIM Varicella zoster virus encodes RIPK cell death Herpesviruses ORF 20 RHIM ORF 20 RHIM forms ZBP 1-expressing HT -29 cells 2020-07-10 17:39:28 Figure https://plos.figshare.com/articles/figure/VZV_infection_inhibits_phosphorylation_of_MLKL_during_TNF-induced_necroptosis_/12642459 <p>(A) Immunofluorescence staining for phosphorylated MLKL (red) and VZV IE62 antigen (green) in mock and VZV infected HT-29 adenocarcinoma cells untreated (DMSO control) or treated with TNF (T; 30 ng/ml), BV-6 (S; 1 μM) and z-VAD-fmk (V; 25 μM) for 7–8 h to induce necroptosis. Following immunostaining cells were counterstained with DAPI (blue). (B) The percentage of cells that were pMLKL positive was determined by randomly imaging 10–20 non-overlapping regions of each slide and manually counting cells from 3 independent experiments. Error bars show standard error of the mean, statistical significance was determined using a one-way ANOVA. Scale bar indicates 20 μm.</p>