10.1371/journal.pcbi.1007902.g003 Sandra Mayr Sandra Mayr Fabian Hauser Fabian Hauser Sujitha Puthukodan Sujitha Puthukodan Markus Axmann Markus Axmann Janett Göhring Janett Göhring Jaroslaw Jacak Jaroslaw Jacak Comparison of IL-1β treated and untreated clot samples. Public Library of Science 2020 platelet clot model sample analysis software platform 2 CALM 3 D localisation microscopy images protein distributions 2 CALM parameter membrane protein distributions 3 D localisation microscopy data 2020-06-30 17:38:01 Figure https://plos.figshare.com/articles/figure/Comparison_of_IL-1_treated_and_untreated_clot_samples_/12591513 <p>(a) and (b) show reconstructed dSTORM images of two clots, untreated (a) and treated with IL-1β (b). In (c) zoomed images of two regions extracted from (a) and (b) are shown. In (d) two populations of clusters from both images with a cluster dimension of 145 nm are visualised. (e) shows the comparison of cluster densities for all given cluster dimensions between the two datasets (1<sup>st</sup>-level statistic). Blue and red lines depict the results of the KS- and WX-test, respectively [<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1007902#pcbi.1007902.ref075" target="_blank">75</a>, <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1007902#pcbi.1007902.ref088" target="_blank">88</a>]. The aggregated p-value between KS and WX tests (black dashed line) remains below the critical p-value area (orange bar) and thus proves the dissimilarity hypothesis for most of the cluster dimensions. (f) shows the aggregated p-values of averaged density and curvature distribution, determined via mean-cross analysis (blue) and the average p-value (green, including confidential intervals: black dashed lines) (<i>sim</i><sub><i>M</i></sub> = 0.07 and <i>sim</i><sub><i>L</i></sub> = 0.06). The table in (g) shows the classification table with MLP neural network classification values for dissimilar and similar samples. The output values of the trained network can be interpreted as <i>a posteriori</i> probability of similarity hypotheses.</p>