Regulated silencing of hStau1 protein in neuroblastoma cells.
Joan Peredo
Patricia Villacé
Juan Ortín
Susana de Lucas
10.1371/journal.pone.0113704.g006
https://plos.figshare.com/articles/figure/_Regulated_silencing_of_hStau1_protein_in_neuroblastoma_cells_/1251673
<p>Cultures of neuroblastoma SH-SY5Y cells were transduced with a lentiviral construct able to express, under a doxicyclin inducible promoter, RFP and an shRNA with a hStau1 specific silencer (siStau) or an empty shRNA as a control (Ctrl). (A) Combined phase-contrast and fluorescence images of cells treated (bottom) or untreated (top) with doxicyclin. Bar scale correspond to 10 µm. (B) Cell extracts were obtained from the cultures described above and analysed by Western-blot using antibodies specific for hStau1 or ß-actin.</p>
2014-11-25 03:06:46
Staufen 1 mRNA targets
dendritic arborisation
Staufen 1.
neuroblastoma cells
translation repression
Staufen 1 complexes
neuroblastoma cell differentiation
Staufen 1
Motor proteins
localised translation
Human Staufen 1 Associates
intracellular localization
Neuronal Cell Differentiation
cell fate