Peredo, Joan Villacé, Patricia Ortín, Juan de Lucas, Susana Analysis of hStau1 complexes and the associated miRNAs in human neuroblastoma cells. <p>Soluble extracts derived from the SH-SY5Y neuroblastoma cell line were filtered on a Sephacryl S-400 column. (A) The various fractions were analysed by Western-blot with antibodies specific for Ago2, RCK/p54 and hStau1. (B) The fractions were grouped in 4 pools: F1 (17, 18), F2 (21,22,23), F3 (26,27,28,29) and F4 (34,35,36 as a negative control). RNA was isolated in each pool and analysed by TaqMan RT-qPCR to quantify the six miRNAs most prevalent in hStau1 complexes. (C) The amounts of miR-124 and miR-9 were determined in the fractions pools F1 to F4 derived from 3 independent filtration experiments. Values are averages and standard deviations and represent the amount of miRNA present in each fraction pool as percentage of total miRNA recovered from F1+F2+F3+F4 pools.</p> Staufen 1 mRNA targets;dendritic arborisation;Staufen 1.;neuroblastoma cells;translation repression;Staufen 1 complexes;neuroblastoma cell differentiation;Staufen 1;Motor proteins;localised translation;Human Staufen 1 Associates;intracellular localization;Neuronal Cell Differentiation;cell fate 2014-11-25
    https://plos.figshare.com/articles/figure/_Analysis_of_hStau1_complexes_and_the_associated_miRNAs_in_human_neuroblastoma_cells_/1251670
10.1371/journal.pone.0113704.g004