Gentiolactone does not inhibit DNA binding of NF-κB. Hidetoshi Yamada Sayaka Kikuchi Tomoki Inui Hideyuki Takahashi Ken-ichi Kimura 10.1371/journal.pone.0113834.g007 https://plos.figshare.com/articles/figure/_Gentiolactone_does_not_inhibit_DNA_binding_of_NF_954_B_/1251356 <p>RAW264.7 cells were cultured for 1 hour with LPS (1 µg/mL) and gentiolactone (250, 500, 1000 µM). Nuclear proteins were extracted and incubated with biotinylated oligonucleotides containing a binding site for NF-κB. Resultant complexes were analyzed by EMSA. The bands indicated by the arrow were quantified. Plotted values represent the mean value ± SD from three independent cultures. Helenalin was used as a positive control.</p> 2014-11-25 02:44:48 lps Murine Macrophages BackgroundGentian roots inhibition compound RAW 264.7 cells inos extract mrna gentiolactone expression Gentiana triflora nf mechanism cox tnf gentian roots