%0 Figure %A Yamada, Hidetoshi %A Kikuchi, Sayaka %A Inui, Tomoki %A Takahashi, Hideyuki %A Kimura, Ken-ichi %D 2014 %T Gentiolactone inhibits LPS-induced TNF-α mRNA expression. %U https://plos.figshare.com/articles/figure/_Gentiolactone_inhibits_LPS_induced_TNF_945_mRNA_expression_/1251351 %R 10.1371/journal.pone.0113834.g003 %2 https://plos.figshare.com/ndownloader/files/1806407 %K lps %K Murine Macrophages BackgroundGentian roots %K inhibition %K compound %K RAW 264.7 cells %K inos %K extract %K mrna %K gentiolactone %K expression %K Gentiana triflora %K nf %K mechanism %K cox %K tnf %K gentian roots %X

RAW264.7 cells were cultured for 4 hours with LPS (100 ng/mL) and 100, 500 or 1000 µM gentiolactone. The mRNA levels of TNF-α (A), iNOS (B) and Cox-2 (C) were measured by real-time PCR. (D) TNF-α protein levels were measured with an ELISA assay. Plotted values represent the mean value ± SD from four independent cultures. *p<0.05, **p<0.01. Helenalin was used as a positive control.

%I PLOS ONE