Comparative Genomics of Transcriptional Regulation of Methionine Metabolism in Proteobacteria
Semen A. Leyn
Inna A. Suvorova
Tatiana D. Kholina
Sofia S. Sherstneva
Pavel S. Novichkov
Mikhail S. Gelfand
Dmitry A. Rodionov
10.1371/journal.pone.0113714
https://plos.figshare.com/articles/dataset/_Comparative_Genomics_of_Transcriptional_Regulation_of_Methionine_Metabolism_in_Proteobacteria_/1248173
<div><p>Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in ∼200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the <i>metX</i> or <i>metZ</i> genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific and genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.</p></div>
2014-11-20 04:44:30
methionine metabolism genes
regulator
sah
Comparative genomics
sam
Proteobacteria Methionine metabolism
genome
dna
novel regulons
transcriptional regulation
regulon contents
transcription factors
riboswitch motifs
Methionine Metabolism
rna
metZ genes
uptake genes
methionine biosynthesis genes
Xanthomonadales group
22 taxonomic groups