Trehalose induced podocyte autophagy in an mTOR independent manner. KangYu-Lin Ahson SaleemMoin Wah ChanKwok Yat-Ming YungBenjamin Ka-Wai LawHelen 2014 <p>mTOR activity was not altered in trehalose-treated podocytes. Podocytes were treated with trehalose (50 mM) for 0, 12, 24, 36, 48 and 60 h. The phosphorylation level of p-mTOR (<b>A</b>) and its substrates p-p70S6K (<b>B</b>) and p-4E-BP1 (<b>C</b>) were measured by Western blotting, nā€Š=ā€Š5, 5 and 6 respectively. However, no significant changes were observed. The representative immunoblot was shown along with the statistical results.</p>