10.1371/journal.pone.0112474.g001
Karina Alves Toledo
Karina
Alves Toledo
Marise Lopes Fermino
Marise
Lopes Fermino
Camillo del Cistia Andrade
Camillo
del Cistia Andrade
Thalita Bachelli Riul
Thalita
Bachelli Riul
Renata Tomé Alves
Renata
Tomé Alves
Vanessa Danielle Menjon Muller
Vanessa
Danielle Menjon Muller
Raquel Rinaldi Russo
Raquel
Rinaldi Russo
Sean R. Stowell
Sean
R. Stowell
Richard D. Cummings
Richard D.
Cummings
Victor Hugo Aquino
Victor
Hugo Aquino
Marcelo Dias-Baruffi
Marcelo
Dias-Baruffi
Lower expression of Gal-1 is correlated with higher viral loads produced by DENV-1-infected cells.
Public Library of Science
2014
dengue hemorrhagic fever
virus production
carbohydrate recognition domain
DENV infection
Exerts Inhibitory Effects
rna
gal
hrGal
Infection Dengue virus
dengue virus type 1
2014-11-13 03:37:40
Figure
https://plos.figshare.com/articles/figure/_Lower_expression_of_Gal_1_is_correlated_with_higher_viral_loads_produced_by_DENV_1_infected_cells_/1240146
<p><b>(A)</b> Gal-1 expression on HMVEC-L, Vero-E6 and ECV-304 cells was assessed by western blot method and normalized by β-actin endogenous control. The relative density of Gal-1 was determined by ImageJ software. <b>(B)</b> HMVEC-L, Vero-E6 and ECV-304 cells (2.5x10<sup>4</sup>) were incubated with DENV-1 (MOI 0.5) for 72 hours at 37°C. At the end of incubation period, the total amounts of viral RNA in the cell-free supernatants were determined by Real-Time PCR, using a standard curve constructed from DENV-1 RNA purified from 1x10<sup>7</sup> PFU (PFU: plate formed units). Results are shown as Viral RNA amount equivalents to PFU/ml±SD from 3 independent assays performed in triplicates. <b>(C)</b> ECV-304 cells were inoculated with DENV-1 (MOI 0.5) or only with medium and cultivated for 72 hours at 37°C. Cells were analyzed for Gal-1 expression by western blot assay. <b>(D)</b> Soluble Gal-1 was detected in the supernatants from cell cultures using ELISA method (N = 3). *p<0.01; **p<0.001.</p>