10.1371/journal.pone.0038941
Yu Hisano
Yu
Hisano
Naoki Kobayashi
Naoki
Kobayashi
Akihito Yamaguchi
Akihito
Yamaguchi
Tsuyoshi Nishi
Tsuyoshi
Nishi
Mouse SPNS2 Functions as a Sphingosine-1-Phosphate Transporter in Vascular Endothelial Cells
Public Library of Science
2012
spns2
functions
sphingosine-1-phosphate
transporter
vascular
endothelial
cells
2012-06-12 01:06:38
Dataset
https://plos.figshare.com/articles/dataset/Mouse_SPNS2_Functions_as_a_Sphingosine_1_Phosphate_Transporter_in_Vascular_Endothelial_Cells/123998
<div><p>Sphingosine-1-phosphate (S1P), a sphingolipid metabolite that is produced inside the cells, regulates a variety of physiological and pathological responses via S1P receptors (S1P1–5). Signal transduction between cells consists of three steps; the synthesis of signaling molecules, their export to the extracellular space and their recognition by receptors. An S1P concentration gradient is essential for the migration of various cell types that express S1P receptors, such as lymphocytes, pre-osteoclasts, cancer cells and endothelial cells. To maintain this concentration gradient, plasma S1P concentration must be at a higher level. However, little is known about the molecular mechanism by which S1P is supplied to extracellular environments such as blood plasma. Here, we show that SPNS2 functions as an S1P transporter in vascular endothelial cells but not in erythrocytes and platelets. Moreover, the plasma S1P concentration of SPNS2-deficient mice was reduced to approximately 60% of wild-type, and SPNS2-deficient mice were lymphopenic. Our results demonstrate that SPNS2 is the first physiological S1P transporter in mammals and is a key determinant of lymphocyte egress from the thymus.</p> </div>