Results from one representative ChIP experiment, which included four antibody concentrations, including two (1 μg, 4 μg) that were not included in subsequent experimental replicates. VolpicelliFloriana De GregorioRoberto PulcranoSalvatore Perrone-CapanoCarla di PorzioUmberto BellenchiGian Carlo 2020 <p>For the experiment represented in this file, there was one experimental sample per condition; cells F27-S42 in the Excel file include data for the pPitx3-I experiment, the 2 μg results from this experiment represent one replicate of the three independent experiments summarized in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0233918#pone.0233918.g001" target="_blank">Fig 4D</a> graph. The raw data for the other replicates are not available. Two potential binding sites for Nurr1 on the Pitx3 promoter were identified, named pPitx3-I and pPitx3-II. Only promoter I (pPitx3-I) is significantly enriched after the ChIP and was assessed in following experiments. Quantification of Pitx3 promoter by qPCR after ChIP. Data obtained with 2 μg antibody are reported in the original <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0233918#pone.0233918.g001" target="_blank">Fig 4D</a>. The efficiency of IP was calculated as follows: (a) ΔC<sub>t</sub> (Ct<sub>IP</sub> − Ct<sub>INPUT</sub>) was calculated considering the abundance of a target DNA sequence (bound or immunoprecipitated, Ct<sub>IP</sub>) relative to input chromatin (Ct<sub>INPUT</sub>); (b) the results were expressed as 2<sup>-ΔCt</sup> or as 2<sup>-ΔCt</sup> x 100 (.xls file column O).</p> <p>(XLSX)</p>