Zheng, Xuan Zhang, Xinyan Ding, Lingling R. Lee, Jeffrey M. Weinberger, Paul S. Dynan, William Age and dose-dependent accumulation of 4-hydroxynonenal in liver tissue. <p>Liver sections were stained with anti-4-hydroxy-2-nonenal (4-HNE) and hematoxylin counterstain as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111362#s2" target="_blank">Materials and Methods</a>. A. Bright-field images showing weak, moderate, or intense anti-4-HNE immunohistochemical staining. Scale bars  = 20 µm. B. Nuance renderings of images from Panel A (yellow, 4-HNE; blue, cell nuclei). C. Quantification of 4-HNE staining based on Nuance analysis. Box plot shows mean and interquartile ranges. Color denotes dose group. Values are normalized to 0 Gy, 250 day group. D. Plot showing correlation between actual and predicted natural logarithm (ln)-transformed 4-HNE values for HZE cohort. Predicted values are based on regression model incorporating age, dose, and dose-squared parameters. Each symbol represents one individual. Shape denotes age group; color denotes dose group using same values as in Panel B. E. Plot showing predicted 4-HNE values as a function of age and HZE dose. F, G. Same as Panels D, E for γ-ray cohorts. Regression model incorporates an age parameter only; dose parameters were non-significant.</p> HZE particle radiation;HZE particle radiation exposure;HZE particle radiation dose;HZE exposure increases cancer risk;PPARGC 1A mRNA;space radiation environment;oxidative stress defense genes;oxidative stress;Vertebrate Laboratory Model Organism Oryzias latipes High charge;tissue;Energy Particle Radiation;lipid peroxidation products;laboratory model organism 2014-11-06
    https://plos.figshare.com/articles/figure/_Age_and_dose_dependent_accumulation_of_4_hydroxynonenal_in_liver_tissue_/1231318
10.1371/journal.pone.0111362.g002