%0 Figure %A Zheng, Xuan %A Zhang, Xinyan %A Ding, Lingling %A R. Lee, Jeffrey %A M. Weinberger, Paul %A S. Dynan, William %D 2014 %T Age and dose-dependent accumulation of 4-hydroxynonenal in liver tissue. %U https://plos.figshare.com/articles/figure/_Age_and_dose_dependent_accumulation_of_4_hydroxynonenal_in_liver_tissue_/1231318 %R 10.1371/journal.pone.0111362.g002 %2 https://plos.figshare.com/ndownloader/files/1782318 %K HZE particle radiation %K HZE particle radiation exposure %K HZE particle radiation dose %K HZE exposure increases cancer risk %K PPARGC 1A mRNA %K space radiation environment %K oxidative stress defense genes %K oxidative stress %K Vertebrate Laboratory Model Organism Oryzias latipes High charge %K tissue %K Energy Particle Radiation %K lipid peroxidation products %K laboratory model organism %X

Liver sections were stained with anti-4-hydroxy-2-nonenal (4-HNE) and hematoxylin counterstain as described in Materials and Methods. A. Bright-field images showing weak, moderate, or intense anti-4-HNE immunohistochemical staining. Scale bars  = 20 µm. B. Nuance renderings of images from Panel A (yellow, 4-HNE; blue, cell nuclei). C. Quantification of 4-HNE staining based on Nuance analysis. Box plot shows mean and interquartile ranges. Color denotes dose group. Values are normalized to 0 Gy, 250 day group. D. Plot showing correlation between actual and predicted natural logarithm (ln)-transformed 4-HNE values for HZE cohort. Predicted values are based on regression model incorporating age, dose, and dose-squared parameters. Each symbol represents one individual. Shape denotes age group; color denotes dose group using same values as in Panel B. E. Plot showing predicted 4-HNE values as a function of age and HZE dose. F, G. Same as Panels D, E for γ-ray cohorts. Regression model incorporates an age parameter only; dose parameters were non-significant.

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