%0 Figure %A Yamanaka, Yuki %A Oshima, Taku %A Ishihama, Akira %A Yamamoto, Kaneyoshi %D 2014 %T Induction of NhaR, GadE, and GadW by YdeO in E. coli. %U https://plos.figshare.com/articles/figure/_Induction_of_NhaR_GadE_and_GadW_by_YdeO_in_E_coli_/1231314 %R 10.1371/journal.pone.0111962.g005 %2 https://plos.figshare.com/ndownloader/files/1782314 %K Escherichia coli Enterobacteria %K YdeO regulon %K acidic conditions %K emsa %K candidate YdeO binding sites %K gade %K GAD system %X

YY5002 (gadE-3xflag) (a), YY5003 (gadW-3xflag) (b), and BW25113, (c) harboring pTrc99A (−) or pYdeO (+), were grown in LB medium until logarithmic phase. After centrifugation the lysate solution was prepared in lysis buffer containing 8 M urea by sonication. The lysates were subjected to western blotting as described in Materials and Methods. Anti-FLAG (SIGMA) and anti-NhaR (Lab preparation) were used for detection of GadE-3xFLAG/GadW-3xFLAG, and NhaR, respectively [A]. The amounts of GadE-3xFLAG, GadW-3xFLAg, and NhaR were represented as the ratio of level of RNA polymerase-α subunit, detected by anti-α (Neoclone) [B].

%I PLOS ONE