Reporter assays for transcriptional regulation by YdeO. YamanakaYuki OshimaTaku IshihamaAkira YamamotoKaneyoshi 2014 <p>[A] YdeO-expression induces the expression of target promoters. YY0201/pLUXnhaR (<i>nhaR-lux</i>), HYAA-JL (<i>hyaA-lacZ</i>), YY1101 (<i>yiiS-lacZ</i>), YY0201/pLUXgadWp (gadW-lux), YY0201/pLUXgadE (<i>gadE-lux</i>), and YY0201/pLUXslpp (<i>slp-lux</i>), and were transformed with pTrc99A (vector, white bar) and pYdeO (<i>ydeO</i>, black bar). Transformants grew until logarithmic phase and β-galactosidase and luciferase activities of cultures were measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111962#s2" target="_blank">Materials and methods</a>. The data show the average of independent eight experiments with standard deviation as the ratio of a vector-transformant. [B] APPC-JL (<i>appC-lacZ</i>) was transformed with pTrc99A (vector) or pYdeO (<i>ydeO</i>). Transformants grew until logarithmic phase and β-galactosidase was measured as decribed in [A]. The data show the average of independent eight experiments with standard deviation as the Miller unit. [C] The <i>ydeO</i> expression induced under anaerobic conditions. The activity of <i>ydeO</i> promoter was measured in YY0101 growing in LB medium with pH 7.2 and 5.5 under aerobic (+) and anaerobic (−) conditions at logarithmic phase.</p>