10.1371/journal.pone.0111962.g003
Yuki Yamanaka
Yuki
Yamanaka
Taku Oshima
Taku
Oshima
Akira Ishihama
Akira
Ishihama
Kaneyoshi Yamamoto
Kaneyoshi
Yamamoto
Reporter assays for transcriptional regulation by YdeO.
Public Library of Science
2014
Escherichia coli Enterobacteria
YdeO regulon
acidic conditions
emsa
candidate YdeO binding sites
gade
GAD system
2014-11-06 04:36:51
Figure
https://plos.figshare.com/articles/figure/_Reporter_assays_for_transcriptional_regulation_by_YdeO_/1231311
<p>[A] YdeO-expression induces the expression of target promoters. YY0201/pLUXnhaR (<i>nhaR-lux</i>), HYAA-JL (<i>hyaA-lacZ</i>), YY1101 (<i>yiiS-lacZ</i>), YY0201/pLUXgadWp (gadW-lux), YY0201/pLUXgadE (<i>gadE-lux</i>), and YY0201/pLUXslpp (<i>slp-lux</i>), and were transformed with pTrc99A (vector, white bar) and pYdeO (<i>ydeO</i>, black bar). Transformants grew until logarithmic phase and β-galactosidase and luciferase activities of cultures were measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111962#s2" target="_blank">Materials and methods</a>. The data show the average of independent eight experiments with standard deviation as the ratio of a vector-transformant. [B] APPC-JL (<i>appC-lacZ</i>) was transformed with pTrc99A (vector) or pYdeO (<i>ydeO</i>). Transformants grew until logarithmic phase and β-galactosidase was measured as decribed in [A]. The data show the average of independent eight experiments with standard deviation as the Miller unit. [C] The <i>ydeO</i> expression induced under anaerobic conditions. The activity of <i>ydeO</i> promoter was measured in YY0101 growing in LB medium with pH 7.2 and 5.5 under aerobic (+) and anaerobic (−) conditions at logarithmic phase.</p>