10.1371/journal.pone.0111962.g003 Yuki Yamanaka Yuki Yamanaka Taku Oshima Taku Oshima Akira Ishihama Akira Ishihama Kaneyoshi Yamamoto Kaneyoshi Yamamoto Reporter assays for transcriptional regulation by YdeO. Public Library of Science 2014 Escherichia coli Enterobacteria YdeO regulon acidic conditions emsa candidate YdeO binding sites gade GAD system 2014-11-06 04:36:51 Figure https://plos.figshare.com/articles/figure/_Reporter_assays_for_transcriptional_regulation_by_YdeO_/1231311 <p>[A] YdeO-expression induces the expression of target promoters. YY0201/pLUXnhaR (<i>nhaR-lux</i>), HYAA-JL (<i>hyaA-lacZ</i>), YY1101 (<i>yiiS-lacZ</i>), YY0201/pLUXgadWp (gadW-lux), YY0201/pLUXgadE (<i>gadE-lux</i>), and YY0201/pLUXslpp (<i>slp-lux</i>), and were transformed with pTrc99A (vector, white bar) and pYdeO (<i>ydeO</i>, black bar). Transformants grew until logarithmic phase and β-galactosidase and luciferase activities of cultures were measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111962#s2" target="_blank">Materials and methods</a>. The data show the average of independent eight experiments with standard deviation as the ratio of a vector-transformant. [B] APPC-JL (<i>appC-lacZ</i>) was transformed with pTrc99A (vector) or pYdeO (<i>ydeO</i>). Transformants grew until logarithmic phase and β-galactosidase was measured as decribed in [A]. The data show the average of independent eight experiments with standard deviation as the Miller unit. [C] The <i>ydeO</i> expression induced under anaerobic conditions. The activity of <i>ydeO</i> promoter was measured in YY0101 growing in LB medium with pH 7.2 and 5.5 under aerobic (+) and anaerobic (−) conditions at logarithmic phase.</p>