PAR<sub>1</sub> agonist-induced G<sub>12/13</sub> signaling is impaired in NCI-H28 cells. Alessandra Fazzini Vanessa D’Antongiovanni Laura Giusti Ylenia Da Valle Federica Ciregia Ilaria Piano Antonella Caputo Anna Maria D’Ursi Claudia Gargini Antonio Lucacchini Maria Rosa Mazzoni 10.1371/journal.pone.0111550.g005 https://plos.figshare.com/articles/figure/_PAR_1_agonist_induced_G_12_13_signaling_is_impaired_in_NCI_H28_cells_/1227677 <p>A, relative levels of RhoA activation in response to thrombin in Met-5A and NCI-H28 cells. B, relative levels of RhoA activation in response to the selective PAR<sub>1</sub>-AP in Met-5A and NCI-H28 cells. Rho A activation was measured in serum and growth factor starved cells using the RhoA G-LISA kit from Cytoskeleton. Data shown are mean ± SEM of three independent experiments performed in triplicate. The differences in RhoA activation between Ctrl (vehicle treated Met-5A or NCI-H28 cells) and agonist-treated cells were significant (*P≤0.05, **P≤0.01, ***P≤0.001) by one-way ANOVA followed by Bonferroni’s multiple comparison test (n = 3).</p> 2014-11-03 03:53:37 plasma membrane localization mesothelioma cell lines dysfunctional PAR 1 Malignant Pleural Mesothelioma Cell Line mesothelioma REN cells PAR 1 cell surface PAR 1 expression