10.1371/journal.pone.0111550.g005 Alessandra Fazzini Alessandra Fazzini Vanessa D’Antongiovanni Vanessa D’Antongiovanni Laura Giusti Laura Giusti Ylenia Da Valle Ylenia Da Valle Federica Ciregia Federica Ciregia Ilaria Piano Ilaria Piano Antonella Caputo Antonella Caputo Anna Maria D’Ursi Anna Maria D’Ursi Claudia Gargini Claudia Gargini Antonio Lucacchini Antonio Lucacchini Maria Rosa Mazzoni Maria Rosa Mazzoni PAR<sub>1</sub> agonist-induced G<sub>12/13</sub> signaling is impaired in NCI-H28 cells. Public Library of Science 2014 plasma membrane localization mesothelioma cell lines dysfunctional PAR 1 Malignant Pleural Mesothelioma Cell Line mesothelioma REN cells PAR 1 cell surface PAR 1 expression 2014-11-03 03:53:37 Figure https://plos.figshare.com/articles/figure/_PAR_1_agonist_induced_G_12_13_signaling_is_impaired_in_NCI_H28_cells_/1227677 <p>A, relative levels of RhoA activation in response to thrombin in Met-5A and NCI-H28 cells. B, relative levels of RhoA activation in response to the selective PAR<sub>1</sub>-AP in Met-5A and NCI-H28 cells. Rho A activation was measured in serum and growth factor starved cells using the RhoA G-LISA kit from Cytoskeleton. Data shown are mean ± SEM of three independent experiments performed in triplicate. The differences in RhoA activation between Ctrl (vehicle treated Met-5A or NCI-H28 cells) and agonist-treated cells were significant (*P≤0.05, **P≤0.01, ***P≤0.001) by one-way ANOVA followed by Bonferroni’s multiple comparison test (n = 3).</p>