Fazzini, Alessandra D’Antongiovanni, Vanessa Giusti, Laura Da Valle, Ylenia Ciregia, Federica Piano, Ilaria Caputo, Antonella D’Ursi, Anna Maria Gargini, Claudia Lucacchini, Antonio Rosa Mazzoni, Maria NCI-H28 cells over-express PAR<sub>1</sub>. <p>A, relative expression levels of PAR<sub>1</sub> mRNA in Met-5A and NCI-H28 cells as determined by real time RT-PCR. B, relative expression levels of PAR<sub>1</sub> protein in primary mesothelial cells, Met-5A, NCI-H28, REN, Ist-Mes2, and Mero-14 cell lines as determined by immunoblot analysis followed by densitometric quantitation. Data are expressed as arbitrary unit (fold increase over Ctrl, Met-5A cells) after normalization by β-actin. Data shown are mean ± SEM of three independent experiments. The differences in PAR<sub>1</sub> expression levels between Ctrl (Met-5A or primary mesothelial cells) and MPM cells were significant (*P≤0.05, ***P≤0.001) by one-way ANOVA followed by Bonferroni’s multiple comparison test (n = 3). C, a representative immunoblot.</p> plasma membrane localization;mesothelioma cell lines;dysfunctional PAR 1;Malignant Pleural Mesothelioma Cell Line;mesothelioma REN cells;PAR 1;cell surface PAR 1 expression 2014-11-03
    https://plos.figshare.com/articles/figure/_NCI_H28_cells_over_express_PAR_1_/1227671
10.1371/journal.pone.0111550.g001