<i>In vivo</i> effect of miR-21 on CNE1 cell growth in nude mice. Hesheng Ou Yumei Li Min Kang 10.1371/journal.pone.0109929.g005 https://plos.figshare.com/articles/figure/_In_vivo_effect_of_miR_21_on_CNE1_cell_growth_in_nude_mice_/1227663 <p>CNE1 cells (10<sup>7</sup> cells/tumor), treated with miR-21 or control (100 nM for 48 h) without transfection reagents, were injected subcutaneously into the right axilla of nude mice. <b>(A)</b> Growth curves for CNE1 cells treated with miR-21 (n = 8) and control (n = 8) obtained by <i>in vivo</i> proliferation assay. P<0.05 for all. <b>(B)</b> Photographs of tumors from miR-21 and control groups. <b>(C)</b> Photographs of mice from miR-21 and control groups. <b>(D)</b> Representative photomicrographs of CNE1 cells and apoptosis in miR-21 and control groups detected by TUNEL assay. <b>(E)</b> Representative photomicrographs of immunohistochemical staining and the relative levels of Bcl-2 and Bax on xenograft tumor sections obtained from mice in miR-21 and control groups. The cytoplasm was counter-stained with hematoxylin (×400).</p> 2014-11-03 03:52:19 NPC cell lines AKT activity vivo assays nasopharyngeal carcinoma Targeting PTEN Gene PTEN mRNA Suppresses Apoptosis flow cytometry DNA fragmentation STAT 3 protein TUNEL assay PTEN tumor suppressor 42 patients NPC tissues STAT 3 Induces Proliferation lymph node metastasis CNE 2 cell lines Cell growth NPC cells luciferase reporter assay NPC cell proliferation mir cell assays transcription factor CNE 1 utr il STAT 3 mRNA