Inácio, Angela Pinho, Joana Matos Pereira, Patrícia Comai, Luca Manuela Coelho, Maria Global Analysis of the Small RNA Transcriptome in Different Ploidies and Genomic Combinations of a Vertebrate Complex – The <em>Squalius alburnoides</em> <div><p>The <em>Squalius alburnoides</em> complex (Steindachner) is one of the most intricate hybrid polyploid systems known in vertebrates. In this complex, the constant switch of the genome composition in consecutive generations, very frequently involving a change on the ploidy level, promotes repetitive situations of potential genomic shock. Previously in this complex, it was showed that in response to the increase in genome dosage, triploids hybrids could regulate gene expression to a diploid state. In this work we compared the small RNA profiles in the different genomic compositions interacting in the complex in order to explore the miRNA involvement in gene expression regulation of triploids. Using high-throughput arrays and sequencing technologies we were able to verify that diploid and triploid hybrids shared most of their sequences and their miRNA expression profiles were high correlated. However, an overall view indicates an up-regulation of several miRNAs in triploids and a global miRNA expression in triploids higher than the predicted from an additive model. Those results point to a participation of miRNAs in the cellular functional stability needed when the ploidy change.</p> </div> rna;transcriptome;ploidies;genomic;combinations;vertebrate 2012-07-18
    https://plos.figshare.com/articles/dataset/Global_Analysis_of_the_Small_RNA_Transcriptome_in_Different_Ploidies_and_Genomic_Combinations_of_a_Vertebrate_Complex_The_Squalius_alburnoides_/122565
10.1371/journal.pone.0041158