10.1371/journal.pone.0041205 Lech Ignatowicz Lech Ignatowicz Jolanta Mazurek Jolanta Mazurek Chaniya Leepiyasakulchai Chaniya Leepiyasakulchai Markus Sköld Markus Sköld Jorma Hinkula Jorma Hinkula Gunilla Källenius Gunilla Källenius Andrzej Pawlowski Andrzej Pawlowski <em>Mycobacterium tuberculosis</em> Infection Interferes with HIV Vaccination in Mice Public Library of Science 2012 interferes hiv vaccination mice 2012-07-23 00:37:41 Dataset https://plos.figshare.com/articles/dataset/_Mycobacterium_tuberculosis_Infection_Interferes_with_HIV_Vaccination_in_Mice/122261 <div><p>Tuberculosis (TB) has emerged as the most prominent bacterial disease found in human immunodeficiency virus (HIV)-positive individuals worldwide. Due to high prevalence of asymptomatic <em>Mycobacterium tuberculosis</em> (<em>Mtb</em>) infections, the future HIV vaccine in areas highly endemic for TB will often be administrated to individuals with an ongoing <em>Mtb</em> infection. The impact of concurrent <em>Mtb</em> infection on the immunogenicity of a HIV vaccine candidate, MultiHIV DNA/protein, was investigated in mice. We found that, depending on the vaccination route, mice infected with <em>Mtb</em> before the administration of the HIV vaccine showed impairment in both the magnitude and the quality of antibody and T cell responses to the vaccine components p24Gag and gp160Env. Mice infected with <em>Mtb</em> prior to intranasal HIV vaccination exhibited reduced p24Gag-specific serum IgG and IgA, and suppressed gp160Env-specific serum IgG as compared to respective titers in uninfected HIV-vaccinated controls. Importantly, in <em>Mtb</em>-infected mice that were HIV-vaccinated by the intramuscular route the virus neutralizing activity in serum was significantly decreased, relative to uninfected counterparts. In addition mice concurrently infected with <em>Mtb</em> had fewer p24Gag-specific IFN-γ-expressing T cells and multifunctional T cells in their spleens. These results suggest that <em>Mtb</em> infection might interfere with the outcome of prospective HIV vaccination in humans.</p> </div>