10.1371/journal.pone.0232476.g006
Gerardo R. Corradi
Gerardo R.
Corradi
Luciana R. Mazzitelli
Luciana R.
Mazzitelli
Guido D. Petrovich
Guido D.
Petrovich
Paula Grenon
Paula
Grenon
Danny M. Sørensen
Danny M.
Sørensen
Michael Palmgren
Michael
Palmgren
Felicitas de Tezanos Pinto
Felicitas
de Tezanos Pinto
Hugo P. Adamo
Hugo P.
Adamo
Phosphoenzyme formation by the pure Spf1p preparation.
Public Library of Science
2020
phosphatase P 5 ATPases
phosphatase Pho 8p
D 487 N
EGTA
P 5 ATPases
Spf 1p P 5A ATPase
P 5A Spf 1p phosphoenzyme
Spf 1p preparation
Spf 1p
ATP hydrolytic activity
contaminant-free Spf 1p preparation
2020-04-30 17:29:26
Figure
https://plos.figshare.com/articles/figure/Phosphoenzyme_formation_by_the_pure_Spf1p_preparation_/12222401
<p>Spf1p (1.5 μg) was phosphorylated under identical conditions as those described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0232476#pone.0232476.g004" target="_blank">Fig 4</a>. <b>Panel A-</b> Acidic gel electrophoresis. <b>Panel B-</b> Coomassie Blue-stained gel. <b>Panel C-</b> Quantitation of EP. Duplicates for each condition are shown. The EP level detected in the EGTA reaction medium was taken as 100%. <i>Error bars</i> show the standard deviation.</p>