10.1371/journal.pone.0232476.g006 Gerardo R. Corradi Gerardo R. Corradi Luciana R. Mazzitelli Luciana R. Mazzitelli Guido D. Petrovich Guido D. Petrovich Paula Grenon Paula Grenon Danny M. Sørensen Danny M. Sørensen Michael Palmgren Michael Palmgren Felicitas de Tezanos Pinto Felicitas de Tezanos Pinto Hugo P. Adamo Hugo P. Adamo Phosphoenzyme formation by the pure Spf1p preparation. Public Library of Science 2020 phosphatase P 5 ATPases phosphatase Pho 8p D 487 N EGTA P 5 ATPases Spf 1p P 5A ATPase P 5A Spf 1p phosphoenzyme Spf 1p preparation Spf 1p ATP hydrolytic activity contaminant-free Spf 1p preparation 2020-04-30 17:29:26 Figure https://plos.figshare.com/articles/figure/Phosphoenzyme_formation_by_the_pure_Spf1p_preparation_/12222401 <p>Spf1p (1.5 μg) was phosphorylated under identical conditions as those described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0232476#pone.0232476.g004" target="_blank">Fig 4</a>. <b>Panel A-</b> Acidic gel electrophoresis. <b>Panel B-</b> Coomassie Blue-stained gel. <b>Panel C-</b> Quantitation of EP. Duplicates for each condition are shown. The EP level detected in the EGTA reaction medium was taken as 100%. <i>Error bars</i> show the standard deviation.</p>