%0 Figure %A Takenaga, Keizo %A Akimoto, Miho %A Koshikawa, Nobuko %A Nagase, Hiroki %D 2020 %T Infiltration of neutrophils and tumor angiogenesis in orthotopic tumors. %U https://plos.figshare.com/articles/figure/Infiltration_of_neutrophils_and_tumor_angiogenesis_in_orthotopic_tumors_/12201575 %R 10.1371/journal.pone.0232230.g005 %2 https://plos.figshare.com/ndownloader/files/22439888 %K modulates IL -33-ST %K mouse pancreatic cancer cell lines %K mouse pancreatic cancer Panc 02 cells %K receptor ST 2L %K pancreatic cancer cells %K Cxcl 3 expression %K colon cancer cells %K CXCL 3 %K mRNA expression levels %K CXCL 3-CXCR axis %K PCR %K pancreatic cancer %K sST 2-expressing mouse pancreatic cancer Panc 02 cells %K tumor microenvironment %K Conclusions Cancer cell-derived sST 2 %K Methods ST 2 %K cancer cell-derived interleukin -33 decoy receptor sST 2 %K IL -33 decoy receptor sST 2 %K orthotopic tumor growth %K sST 2 secretion %K MPO %K tumor growth %K shRNA-resistant sST 2 mRNA %K sST 2 %K sST 2 expression %K ELISA %K IL -33 knockout mice %K SB %K C 57BL mice %K murine pancreatic cancer model Background Proinflammatory interleukin -33 %K tumor tissues %K IL -33-ST %X

(A) The amount of CXCL3 in Panc02-shCont (n = 9) and Panc02-sh#5 (n = 9) tumor tissues. (B) IHC analysis of MPO+ neutrophil infiltration in Panc02-shCont and Panc02-sh#5 tumors. Bar: 50 μm. (C) The number of MPO+ cells per field (×400 magnification) in Panc02-shCont (n = 9 fields of 2 tissue sections) and Panc02-sh#5 tumors (n = 12 fields of 2 tissue sections). (D) RT-qPCR analysis of expression of the neutrophil marker gene Ly6G in Panc02-shCont (n = 5 tumors) and Panc02-sh#5 tumors (n = 6 tumors). (E) The number of F4/80+ cells per field (×400 magnification) in Panc02-shCont (n = 10 fields of 5 tissue sections) and Panc02-sh#5 tumors (n = 12 fields of 4 tissue sections). (F) The number of CD20+ cells per field (×400 magnification) in Panc02-shCont (n = 9 fields of 3 tissue sections) and Panc02-sh#5 tumors (n = 10 fields of 3 tissue sections). (G, H) Angiogenesis in Panc02-shCont and Panc02-sh#5 tumors. G: CD31 staining. Bar: 50 μm. H: Vessel density. Panc02-shCont (n = 11 fields) and Panc02-sh#5 cells (n = 14 fields). The data are shown as the mean ± SD. *P<0.05. ns, not significant.

%I PLOS ONE